Abstract The ability to examine bone vascularity using micro-computed tomography following vessel perfusion with Microfil® and to subsequently perform histologic bone analysis in the same specimen would provide an efficient method by which the vascular and cellular environment of bone can be examined simultaneously. The purpose of this report is to determine if the administration of Microfil precludes accurate histologic assessment of bone quality via osteocyte count and empty lacunae count. Sprague–Dawley rats (n = 6) underwent perfusion with Microfil. Left hemi-mandibles were harvested, decalcified, and underwent vascular analysis via micro-computed tomography prior to sectioning and staining with Gomori’s trichrome. Quantitative histomorphometric evaluation was performed. Ninety-five percent confidence intervals (CIs) were used to determine statistical differences from an established set of controls (n = 12). Histologic analyses were successfully performed on specimens that had been perfused. Quantitative measures of bone cellularity of perfused versus control specimens revealed no statistical difference in osteocyte count per high-power field (95·33 versus 94·66; 95% CI: −7·64 to 6·30) or empty lacunae per high-power field (2·73 versus 1·89; 95% CI: −1·81 to 0·13). A statistical validation is reported that allows histologic analysis of cell counts in specimens which had been perfused with Microfil.

中文翻译：

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Microfil 血管灌注后组织学骨分析的验证

摘要 使用 Microfil® 灌注血管后使用显微计算机断层扫描检查骨血管，随后在同一标本中进行组织学骨分析的能力将提供一种有效的方法，通过该方法可以同时检查骨骼的血管和细胞环境。本报告的目的是确定使用 Microfil 是否会妨碍通过骨细胞计数和空腔计数对骨质量进行准确的组织学评估。Sprague-Dawley 大鼠（n = 6）接受 Microfil 灌注。收获左半下颌骨，脱钙，并在切片和 Gomori 三色染色之前通过显微计算机断层扫描进行血管分析。进行了定量组织形态计量学评估。95% 的置信区间 (CI) 用于确定与一组既定对照 (n = 12) 的统计差异。对已灌注的标本成功进行了组织学分析。灌注标本与对照标本的骨细胞数量的定量测量显示，每个高倍视野的骨细胞计数（95·33 对 94·66；95% CI：-7·64 至 6·30）或每个高倍视野的空腔隙没有统计学差异。功率场（2·73 对 1·89；95% CI：-1·81 到 0·13）。据报道，统计验证允许对已用 Microfil 灌注的样本中的细胞计数进行组织学分析。灌注标本与对照标本的骨细胞数量的定量测量显示，每个高倍视野的骨细胞计数（95·33 对 94·66；95% CI：-7·64 至 6·30）或每个高倍视野的空腔隙没有统计学差异。功率场（2·73 对 1·89；95% CI：-1·81 到 0·13）。据报道，统计验证允许对已用 Microfil 灌注的样本中的细胞计数进行组织学分析。灌注标本与对照标本的骨细胞数量的定量测量显示，每个高倍视野的骨细胞计数（95·33 对 94·66；95% CI：-7·64 至 6·30）或每个高倍视野的空腔隙没有统计学差异。功率场（2·73 对 1·89；95% CI：-1·81 到 0·13）。据报道，统计验证允许对已用 Microfil 灌注的样本中的细胞计数进行组织学分析。