Abstract For researchers seeking to collect spinal cord samples from mice and rats while avoiding acid decalcification, few options are available. Laminectomy is the standard method which yields high quality samples, yet is time consuming and technically difficult. Ejection of the cord from the vertebral column is another technique commonly used; however, the literature suggests that this method can damage the spinal tissues and is typically avoided when histology of samples is the desired endpoint. Here, we describe an optimized method for ejection of spinal cords from rats and mice, and compare histological quality of these samples with those collected via laminectomy. Our results show that ejection can yield high quality spinal cord samples and may be suitable for use as an alternative to laminectomy.

中文翻译：

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标准椎板切除术与优化弹射方法去除大鼠和小鼠脊髓的比较

摘要 对于寻求从小鼠和大鼠收集脊髓样本同时避免酸脱钙的研究人员来说，可用的选择很少。椎板切除术是产生高质量样本的标准方法，但耗时且技术难度大。从脊柱中取出脊髓是另一种常用的技术；然而，文献表明这种方法会损伤脊柱组织，当样本的组织学是所需的终点时，通常会避免这种方法。在这里，我们描述了一种从大鼠和小鼠中射出脊髓的优化方法，并将这些样本的组织学质量与通过椎板切除术收集的样本进行比较。我们的结果表明，弹射可以产生高质量的脊髓样本，并且可能适合用作椎板切除术的替代方法。