Abstract Methods relative to the staining of tissues using hematoxylin and eosin (H&E) have largely not evolved beyond linear batch staining processes. The batching of slides in the histopathology laboratory inherently leads to the sharing of the various reagents among those specimens being processed through the baths. Studies analyzing the effects of reagent sharing during the common H&E linear staining method are limited. This study assessed rates of extraneous tissue contamination found in selected stainer bath containers from the deparaffinization portion of the H&E linear staining procedure. The impact of common mitigation strategies on those rates of contamination was evaluated.

中文翻译：

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染色浴污染的测量和常见缓解策略的评估

摘要 与使用苏木精和伊红 (H&E) 染色组织相关的方法在很大程度上没有超越线性批量染色过程。组织病理学实验室中的载玻片批处理固有地导致在通过浴处理的那些样本之间共享各种试剂。在常见的 H&E 线性染色方法中分析试剂共享影响的研究是有限的。本研究评估了在 H&E 线性染色程序的脱蜡部分选定的染色浴容器中发现的外来组织污染率。评估了常见缓解策略对这些污染率的影响。