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Polyhexanide and hydrogen peroxide inhibit proteoglycan synthesis of human chondrocytes
Journal of Histotechnology ( IF 0.6 ) Pub Date : 2011-03-01 , DOI: 10.1179/014788811x12949268296121
Eric Röhner 1 , Paula Hoff , Tobias Winkler , Philipp von Roth , Jörn Bengt Seeger , Carsten Perka , Georg Matziolis
Affiliation  

Abstract The use of local antiseptics is a common method in septic joint surgery. We tested polyhexanide and hydrogen peroxide, two of the most frequently used antiseptics with high efficacy and low toxicity. The purpose of this study was to evaluate the effects of both antiseptics on the extracellular cartilaginous matrix synthesis of human chondrocytes. Chondrocytes were isolated from donated human knee joints, embedded in alginate beads, and incubated for 10 and 30 minutes with polyhexanide (0·04%), hydrogen peroxide (3%), or phosphate-buffered saline (PBS) for control. Cartilaginous matrix production was quantified through light microscopic analysis of Alcian blue staining. Cell number and morphology were detected by histological analysis. Chondrocytes showed a decreased intensity of blue colouring after antiseptic treatment versus PBS. In contrast to that, neither the cell number per view field nor the cell morphology differed between the groups. Polyhexanide has more toxic potential than hydrogen peroxide. Based on the fact that the cell number and morphology was not altered by the substances at the examined concentrations, the lower intensity of Alcian blue staining of treated chondrocytes indicates a decreased cartilage-specific matrix synthesis by polyhexanide more than by hydrogen peroxide and control.

中文翻译:

聚己酸和过氧化氢抑制人软骨细胞的蛋白多糖合成

摘要 使用局部消毒剂是化脓性关节手术的常用方法。我们测试了聚己酸和过氧化氢,这是两种最常用的高效低毒防腐剂。本研究的目的是评估两种防腐剂对人软骨细胞细胞外软骨基质合成的影响。从捐赠的人类膝关节中分离出软骨细胞,包埋在藻酸盐珠中,并与作为对照的聚己酰胺 (0·04%)、过氧化氢 (3%) 或磷酸盐缓冲盐水 (PBS) 一起孵育 10 和 30 分钟。通过阿尔辛蓝染色的光学显微镜分析来量化软骨基质的产生。通过组织学分析检测细胞数量和形态。与 PBS 相比,在防腐处理后,软骨细胞显示蓝色强度降低。与此相反,各组之间的每个视野的细胞数和细胞形态都没有差异。聚己内酯比过氧化氢具有更大的潜在毒性。基于所检测浓度的物质未改变细胞数量和形态这一事实,处理过的软骨细胞的阿尔辛蓝染色强度较低表明聚己内酯比过氧化氢和对照更能降低软骨特异性基质合成。
更新日期:2011-03-01
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