Glutamate is considered to be responsible for the pathogenesis of many neurodegenerative diseases. Reactive oxygen species (ROS) production is considered to be involved in the glutamate-induced apoptosis process. In this study, we investigated the neuroprotective effects of Rheum turkestanicum in the glutamate-induced rat pheochromocytoma (PC12 cells) and mouse neuroblastoma (N2a) cell lines. Rutin as an antioxidant was used as positive control. Glutamate cytotoxicity was accompanied by an increment of malondialdehyde (MDA) content, ROS generation and apoptosis induction. However, pretreatment with the root extract of R. turkestanicum significantly reduced MDA content, ROS generation and apoptotic cell death. Also rutin at a dose of 100 µM reduced ROS production and protected against glutamate toxicity. Also the quantification of rutin in R. turkestanicum extract was achieved and was about 0.11% ± 0.01 w/w. All these findings indicated that R. turkestanicum protected PC12 and N2a cells against glutamate-induced oxidative cell death and apoptosis and might raise the possibility of R. turkestanicum usage as a neuroprotective agent.

中文翻译：

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大黄大黄可降低PC12和N2a细胞系中的谷氨酸毒性。

谷氨酸被认为是许多神经退行性疾病的发病机理。活性氧（ROS）的产生被认为与谷氨酸诱导的细胞凋亡过程有关。在这项研究中，我们调查了大黄大黄对谷氨酸诱导的大鼠嗜铬细胞瘤（PC12细胞）和小鼠神经母细胞瘤（N2a）细胞系的神经保护作用。芦丁作为抗氧化剂被用作阳性对照。谷氨酸的细胞毒性伴随着丙二醛（MDA）含量的增加，ROS的产生和细胞凋亡的诱导。但是，用土库曼斯坦根的根提取物进行预处理可显着降低MDA含量，ROS生成和凋亡细胞死亡。芦丁的剂量也为100 µM，可降低ROS的产生并防止谷氨酸的毒性。同样是R中芦丁的定量。获得turkestanicum提取物，约为0.11％±0.01 w / w。所有这些发现表明，土库曼斯坦草保护PC12和N2a细胞免受谷氨酸诱导的氧化性细胞死亡和凋亡，并可能增加土库曼斯坦草用作神经保护剂的可能性。