INTRODUCTION Adult neurogenesis includes proliferation and differentiation of progenitor cells as well as their migration and maturation. In the adult human brain, two neurogenic regions, the hippocampal dentate gyrus (DG) and the subventricular zone (SVZ) of lateral ventricles, have been identified. In the dentate gyrus, three types of transcriptionally active cells and in the subventricular zone, four types of transcriptionally active cells, including GFAP-positive neural stem cells (NSCs), have been differentiated. MATERIAL AND METHODS The aim of the study was to identify and compare density of neurogenic cells between two study groups of patients (7 men, 7 women, mean age 70 ± 6.03) with ischemic stroke and with hemorrhage (6 men, 2 women, mean age 64.75 ± 12.23) and the control group of patients (6 men, 2 women, mean age 64 ± 10.95) free of neuropathologic changes who died suddenly within less than 10 min. RESULTS In both groups, in the hippocampal dentate gyrus and in the subventricular zone of lateral ventricles, the presence of single GFAP-positive neural stem cells and the transcriptionally active cells labelled with phosphorylated histone H3Ser-10 (p-Histone H3Ser-10)/neural progenitor cells (NPCs), was observed. The quantitative analysis of cells with p-Histone H3Ser-10 expression in the hippocampal DG revealed significant differences between the hemorrhage and control groups (p = 0.001, test t). However, in the SVZ, it showed a statistically significant decrease in the density of transcriptionally active cells in the group of patients with ischemic stroke (p = 0.001, test t). A distinct decrease in the density of transcriptionally active cells, proportional to the length of the patients' hospitalization, was observed. CONCLUSIONS Hypoxia belongs to pathomechanic factors responsible for ischemic stroke, which can induce neurogenesis. However, hypoxia along with ischemia and other factors implicated in ischemic stroke, such as the patient's age or duration of ischemia can have a decisive influence on the decrease in the density of transcriptionally active cells in this pathologic process.

中文翻译：

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出血和缺血性中风后成人大脑的神经发生。

引言成人神经发生包括祖细胞的增殖和分化，以及它们的迁移和成熟。在成人的大脑中，已经确定了两个神经源性区域，即侧脑室的海马齿状回（DG）和脑室下区域（SVZ）。在齿状回中，已分化出三种类型的转录活性细胞，在脑室下区，已分化出包括GFAP阳性神经干细胞（NSC）在内的四种类型的转录活性细胞。材料和方法本研究的目的是鉴定和比较两个缺血性卒中和出血患者（7名男性，2名女性，平均）的两个研究组（7名男性，7名女性，平均年龄70±6.03）之间的神经源性细胞密度。年龄64.75±12.23）和对照组患者（男6例，女2例，平均年龄64±10）。95）没有神经病理学改变的人在不到10分钟的时间内突然死亡。结果在两组中，在海马齿状回和侧脑室的脑室下区域，均存在单个GFAP阳性神经干细胞和被磷酸化组蛋白H3Ser-10（p-Histone H3Ser-10）标记的转录活性细胞。观察到神经祖细胞（NPC）。对海马DG中具有p-组蛋白H3Ser-10表达的细胞进行的定量分析显示，出血组与对照组之间存在显着差异（p = 0.001，测试t）。但是，在SVZ中，缺血性中风患者组的转录活性细胞密度在统计学上显着降低（p = 0.001，测试t）。转录活性细胞密度明显降低，观察到与患者住院时间成正比。结论缺氧属于缺血性中风的病理机制因素，可诱导神经发生。但是，缺氧以及缺血和缺血性中风所牵涉的其他因素（例如患者的年龄或缺血持续时间）可能对该病理过程中转录活性细胞密度的降低具有决定性影响。