Calcium-dependent protein kinases (CDPKs) play vital roles in the regulation of plant growth, development, and tolerance to various abiotic stresses. However, little information is available for this gene family in banana. In this study, 44 CDPKs were identified in banana and were classified into four groups based on phylogenetic, gene structure, and conserved motif analyses. The majority of MaCDPKs generally exhibited similar expression patterns in the different tissues. Transcriptome analyses revealed that many CDPKs showed strong transcript accumulation at the early stages of fruit development and postharvest ripening in both varieties. Interaction network and co-expression analysis further identified some CDPKs-mediated network that was potentially active at the early stages of fruit development. Comparative expression analysis suggested that the high levels of CDPK expression in FJ might be related to its fast ripening characteristic. CDPK expression following the abiotic stress treatments indicated a significant transcriptional response to osmotic, cold, and salt treatment, as well as differential expression profiles, between BX and FJ. The findings of this study elucidate the transcriptional control of CDPKs in development, ripening, and the abiotic stress response in banana. Some tissue-specific, development/ripening-dependent, and abiotic stress-responsive candidate MaCDPK genes were identified for further genetic improvement of banana.

中文翻译：

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CDPK基因家族的鉴定，表达和相互作用网络分析表明，它们参与了香蕉的发育，成熟和非生物胁迫反应。

钙依赖性蛋白激酶（CDPK）在调节植物生长，发育以及对各种非生物胁迫的耐受性中起着至关重要的作用。但是，有关香蕉中该基因家族的信息很少。在这项研究中，在香蕉中鉴定出44种CDPK，并根据系统发育，基因结构和保守基序分析将其分为四类。大多数MaCDPK在不同组织中通常表现出相似的表达模式。转录组分析显示许多CDPK在两个品种的果实发育和收获后成熟的早期显示出强大的转录本积累。相互作用网络和共表达分析进一步确定了一些CDPKs介导的网络，该网络在果实发育的早期可能是活跃的。比较表达分析表明，FJ中高水平的CDPK表达可能与其快速成熟特性有关。CDPK非生物胁迫处理后的表达表明BX和FJ之间对渗透，冷和盐处理具有明显的转录反应，以及差异表达谱。这项研究的发现阐明了香蕉中CDPKs在发育，成熟和非生物胁迫反应中的转录调控。一些组织特异性，发育/成熟依赖和非生物胁迫响应候选人MaCDPK基因被确定为香蕉的进一步遗传改良。