BACKGROUND/AIMS New antivirals are needed to supplement or replace currently used drugs. The aim of this study was to evaluate the antiviral activity of synthetic peptides derived from physiological proteins. METHODS Vero cell monolayers were infected with herpes simplex virus 1, vesicular stomatitis virus, adenovirus, and coxsackievirus B5 strains in the presence of different concentrations of the selected peptides and viral yield was determined by plaque reduction assays to evaluate the antiviral activity of the peptides. Virucidal activity was evaluated by determining the residual infectivity of viral suspensions treated for 1 h with the peptides at the same concentrations as in the viral yield assays. RESULTS Among the investigated peptides, the killer peptide proved to exert a considerable antiviral activity against herpes simplex virus, attributable to a direct effect on virus particles, while its derivative K10S showed to be effective against the four investigated virus strains only at the highest concentration tested, yet, the inhibitory effects were only partial. CONCLUSION Overall, initial evidence is provided on the antiviral activity of several peptides, as well as of their derivatives. Further investigation is warranted to ascertain the mechanism of action in order to develop new potential antiviral drugs.

中文翻译：

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源自生理蛋白的合成肽的抗病毒活性。

背景/目的需要新的抗病毒剂来补充或替代当前使用的药物。这项研究的目的是评估源自生理蛋白的合成肽的抗病毒活性。方法在不同浓度的所选肽存在下，用单纯疱疹病毒1，水泡性口腔炎病毒，腺病毒和柯萨奇病毒B5株感染Vero细胞单层，并通过噬斑减少法测定病毒产量，以评估该肽的抗病毒活性。通过确定用与病毒产量测定法相同浓度的肽处理1小时的病毒悬浮液的残留感染性来评估杀病毒活性。结果在所研究的肽中，杀伤肽被证明对单纯疱疹病毒具有相当大的抗病毒活性，这归因于对病毒颗粒的直接作用，而其衍生物K10S仅在测试的最高浓度下才显示出对四种所研究病毒株的有效作用，但抑制作用仅是部分的。结论总体而言，提供了一些肽及其衍生物的抗病毒活性的初步证据。为了开发新的潜在抗病毒药物，有必要进行进一步的研究以确定作用机理。