Engrailed (En) has an important role in neuronal development in vertebrates and invertebrates. In adult Drosophila, although En expression persists throughout adulthood, a detailed description of its expression in sensory neurons has not been made. In this study, en-GAL4 was used to drive UAS-CD8::GFP expression and the projections of sensory neurons were examined with confocal microscopy. En protein expression was confirmed using immunocytochemistry. In the antenna, En is present in subsets of Johnston's organ neurons and of olfactory neurons. En-driven GFP is expressed in axons projecting to 18 identified olfactory glomeruli, originating from basiconic, trichoid and coeloconic sensilla. In most cases both neurons of a sensillum express En. En expression overlaps with that of Acj6, another transcription factor. En-driven GFP is also expressed in a subset of maxillary palp olfactory neurons and in all mechanosensory and gustatory sensilla in the posterior compartment of the labial palps. In the legs and halteres, en-driven GFP is expressed in only a subset of the sensory neurons of different modalities that arise in the posterior compartment. Finally, en-driven GFP is expressed in a single multidendritic sensory neuron in each abdominal segment.

中文翻译：

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成年果蝇感觉神经元亚群中的嵌入表达：增强子陷阱研究。

Engrailed (En) 在脊椎动物和无脊椎动物的神经元发育中具有重要作用。在成年果蝇中，虽然 En 表达在整个成年期持续存在，但尚未对其在感觉神经元中的表达进行详细描述。在这项研究中，en-GAL4 用于驱动 UAS-CD8::GFP 表达，并用共聚焦显微镜检查感觉神经元的投影。使用免疫细胞化学确认En蛋白表达。在天线中，En 存在于约翰斯顿器官神经元和嗅觉神经元的子集中。En 驱动的 GFP 在轴突中表达，轴突投射到 18 个已识别的嗅觉肾小球，起源于基本、三线体和腔体感受器。在大多数情况下，感受器的两个神经元都表达 En。En 表达与另一种转录因子 Acj6 的表达重叠。En 驱动的 GFP 也在上颌触须嗅觉神经元的一个子集中表达，以及在唇触后隔室的所有机械感觉和味觉感受器中表达。在腿和露背中，en-driven GFP 仅在后隔室中出现的不同方式的感觉神经元的一个子集中表达。最后，en-driven GFP 在每个腹部节段的单个多树突感觉神经元中表达。