当前位置: X-MOL 学术Med. Vet. Entomol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti.
Medical and Veterinary Entomology ( IF 1.6 ) Pub Date : 2019-05-23 , DOI: 10.1111/mve.12384
T Chouin-Carneiro 1 , T H Ant 2, 3 , C Herd 2, 3 , F Louis 1 , A B Failloux 1 , S P Sinkins 2, 3
Affiliation  

Transinfections of the maternally transmitted endosymbiont Wolbachia pipientis can reduce RNA virus replication and prevent transmission by Aedes aegypti, and also have the capacity to invade wild-type populations, potentially reaching and maintaining high infection frequencies. Levels of virus transmission blocking are positively correlated with Wolbachia intracellular density. Despite reaching high densities in Ae. aegypti, transinfections of wAlbA, a strain native to Aedes albopictus, showed no blocking of Semliki Forest Virus in previous intrathoracic injection challenges. To further characterize wAlbA blocking in Ae. aegypti, adult females were intrathoracically challenged with Zika (ZIKV) and dengue viruses, and then fed a ZIKV-containing bloodmeal. No blocking was observed with either virus when challenged by intrathoracic injection. However, when ZIKV was delivered orally, wAlbA-infected females showed a significant reduction in viral replication and dissemination compared with uninfected controls, as well as a complete absence of virus in saliva. Although other Wolbachia strains have been shown to cause more robust viral blocking in Ae. aegypti, these findings demonstrate that, in principle, wAlbA could be used to reduce virus transmission in this species. Moreover, the results highlight the potential for underestimation of the strength of virus-blocking when based on intrathoracic injection compared with more natural oral challenges.

中文翻译:

沃尔巴克氏体 wAlbA 菌株可阻断寨卡病毒在埃及伊蚊中的传播。

母体传播的内共生体 Wolbachia pipientis 的转染可以减少 RNA 病毒复制并防止埃及伊蚊传播,并且还具有侵入野生型种群的能力,可能达到并保持高感染频率。病毒传播阻断水平与 Wolbachia 细胞内密度呈正相关。尽管在 Ae 中达到了高密度。aegypti,白纹伊蚊原生菌株 wAlbA 的转染显示在之前的胸腔内注射挑战中没有阻断 Semliki Forest 病毒。进一步表征 Ae 中的 wAlbA 阻断。在埃及,成年雌性被胸腔内感染寨卡病毒 (ZIKV) 和登革热病毒,然后喂食含有寨卡病毒的血粉。当通过胸内注射攻击时,没有观察到任何一种病毒的阻断。然而,当口服 ZIKV 时,与未感染的对照组相比,受 wAlbA 感染的女性的病毒复制和传播显着减少,并且唾液中完全没有病毒。尽管其他 Wolbachia 菌株已被证明在 Ae 中引起更强大的病毒阻断。埃及伊蚊,这些发现表明,原则上,wAlbA 可用于减少该物种的病毒传播。此外,结果强调了与更自然的口服挑战相比,基于胸腔内注射的病毒阻断强度可能被低估。尽管其他 Wolbachia 菌株已被证明在 Ae 中引起更强大的病毒阻断。埃及伊蚊,这些发现表明,原则上,wAlbA 可用于减少该物种的病毒传播。此外,结果强调了与更自然的口服挑战相比,基于胸腔内注射的病毒阻断强度可能被低估。尽管其他 Wolbachia 菌株已被证明在 Ae 中引起更强大的病毒阻断。埃及伊蚊,这些发现表明,原则上,wAlbA 可用于减少该物种的病毒传播。此外,结果强调了与更自然的口服挑战相比,基于胸腔内注射的病毒阻断强度可能被低估。
更新日期:2019-05-23
down
wechat
bug