ABSTRACT Genome-wide mapping of lamin-B1-genome interactions has shown that gene-poor and transcriptionally inactive genomic regions are associated with the nuclear lamina. Numerous studies have suggested that lamins, the major structural components of the nuclear lamina, play a role in global chromatin organization and gene expression. How lamins could influence the 3D genome organization and transcription from the nuclear periphery has, however, remained unclear. Our recent studies showed that lamins differentially regulate distinct lamina-associated chromatin domains (LADs) at the nuclear periphery, which can in turn influence global 3D genome organization and gene expression. In this Extra View, we discuss how by using various genomics tools, it has become possible to reveal the functions of lamins in orchestrating 3D genome organization and gene expression. Abbreviations: 3D: three dimensional; LAD: lamina-associated chromatin domain; 3C: Chromosome Conformation Capture; TAD: topologically associated domain; HiLands: Histone and lamina landscape; NL: nuclear lamina; mESC: mouse embryonic stem cell; DamID: DNA adenine methyltransferase identification;

中文翻译：

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lamins 在 3D 基因组组织和全局基因表达中的作用

摘要 核纤层蛋白 B1 基因组相互作用的全基因组图谱表明，基因贫乏和转录不活跃的基因组区域与核纤层相关。许多研究表明，核纤层蛋白是核层的主要结构成分，在整体染色质组织和基因表达中发挥作用。然而，核纤层蛋白如何影响核外围的 3D 基因组组织和转录仍不清楚。我们最近的研究表明，核纤层蛋白在核外围对不同的核纤层相关染色质域 (LAD) 进行差异调节，进而影响全球 3D 基因组组织和基因表达。在这个额外的视图中，我们讨论如何使用各种基因组学工具，揭示 lamins 在协调 3D 基因组组织和基因表达方面的功能已经成为可能。缩写： 3D：三维；LAD：层相关染色质结构域；3C：染色体构象捕获；TAD：拓扑关联域；HiLands：组蛋白和层状景观；NL：核层；mESC：小鼠胚胎干细胞；DamID：DNA腺嘌呤甲基转移酶鉴定；