ABSTRACT To investigate the potential cytotoxicity of radiofrequency (RF) radiation on central nervous system, rat pheochromocytoma (PC12) cells were exposed to 2.856 GHz RF radiation at a specific absorption rate (SAR) of 4 W/kg for 8 h a day for 2 days in 35 mm Petri dishes. During exposure, the real-time variation of the culture medium temperature was monitored in the first hour. Reactive oxygen species (ROS) production, intracellular Ca2+ concentration, and cell apoptosis rate were assessed immediately after exposure by flow cytometry. The results showed that the medium temperature raised about 0.93 °C, but no significant changes were observed in apoptosis, ROS levels or intracellular Ca2+ concentration after treatment. Although several studies suggested that RF radiation does indeed cause neurological effects, this study presented inconsistent results, indicating that 2.856 GHz RF radiation exposure at a SAR of 4 W/kg does not have a dramatic impact on PC12 cells, and suggests the need for further investigation on the key cellular endpoints of other nerve cells after exposure to RF radiation.

中文翻译：

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2.856 GHz 的射频辐射不影响神经元样 PC12 细胞中的关键细胞终点

摘要 为了研究射频 (RF) 辐射对中枢神经系统的潜在细胞毒性，将大鼠嗜铬细胞瘤 (PC12) 细胞暴露于 2.856 GHz 射频辐射，特定吸收率 (SAR) 为 4 W/kg，持续 8 公顷，每天持续 2 天在 35 毫米培养皿中。在暴露期间，在第一小时监测培养基温度的实时变化。暴露后立即通过流式细胞术评估活性氧 (ROS) 产生、细胞内 Ca2+ 浓度和细胞凋亡率。结果表明，处理后培养基温度升高约0.93℃，但细胞凋亡、ROS水平或细胞内Ca2+浓度均无明显变化。尽管一些研究表明射频辐射确实会导致神经系统的影响，