To characterise Linc00518 expression in prostate cancer and elucidate the potential mechanistic involvement in paclitaxel resistance, the relative expression of Linc00518 and miR‐216b‐5p was determined by real‐time PCR. The regulatory effect of miR‐216b‐5p on either Linc00518 or GATA6 was interrogated with luciferase reporter assay. The endogenous GATA6 protein was analysed by Western blotting. The cell viability was measured by MTT assay and IC50 of paclitaxel was calculated through cell counting.

中文翻译：

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长链非编码 RNA Linc00518 通过隔离 miR-216b-5p 促进人前列腺癌的紫杉醇抗性

为了表征前列腺癌中的 Linc00518 表达并阐明紫杉醇耐药性的潜在机制，通过实时 PCR 确定了 Linc00518 和 miR-216b-5p 的相对表达。miR-216b-5p 对 Linc00518 或 GATA6 的调节作用通过荧光素酶报告基因检测进行了询问。通过蛋白质印迹分析内源性 GATA6 蛋白。通过MTT法测定细胞活力，通过细胞计数计算紫杉醇的IC50。