Aflatoxin M₁ (AFM1) is a 2B Group human carcinogens agent and introduces into the human diet by milk consumption. In the present study 257 raw, 143 pasteurized and 61 UHT milk samples were collected from markets in 117 cities throughout Iran, during winter 2017 and AFM1 contents were determined using a HPLC-FLD method after immunoaffinity clean-up. Mean recovery rates and relative standard deviations of the method were 90.6 ± 5.7%. AFM1 was detected in 252 of 461 (54.7%) samples (mean 31.3 ± 8.8 ng/L), ranging from <10 to 150 ng/L. Three samples (0.6%) exceeded the Iranian maximum limit of 100 ng/L. The mean AFM1 content was significantly higher in raw milk samples obtained from humid climate areas. The extrapolated concentration of AFB1 in dairy cattle feed based on the AFM1 value of milk was 1.96 µg/kg. The AFM1 contamination of milk seems not to be a serious public health concern in Iran.

黄曲霉毒素M ₁（AFM1）是2B类人类致癌剂，可通过食用牛奶引入人类饮食。在本研究中，2017年冬季从伊朗117个城市的市场中收集了257份原始，143份巴氏杀菌和61份UHT牛奶样品，并在进行免疫亲和净化后使用HPLC-FLD方法测定了AFM1含量。该方法的平均回收率和相对标准偏差为90.6±5.7％。在461个（54.7％）样品中的252个（平均31.3±8.8 ng / L）中检测到AFM1，范围从<10到150 ng / L。三个样品（0.6％）超过了伊朗最大限量100 ng / L。从潮湿气候地区获得的原奶样品中的平均AFM1含量明显更高。根据牛奶的AFM1值，奶牛饲料中AFB1的外推浓度为1.96 µg / kg。