BACKGROUND Early inflammatory processes may play an important role in the development of early brain injury (EBI) after subarachnoid hemorrhage (SAH). Experimental studies suggest that anti-inflammatory and membrane-stabilizing drugs might have beneficial effects, although the underlying mechanisms are not fully understood. The aim of this study was to investigate the effect of early treatment with methylprednisolone and minocycline on cerebral perfusion and EBI after experimental SAH. METHODS Male Sprague-Dawley rats were subjected to SAH using the endovascular filament model. 30 minutes after SAH, they were randomly assigned to receive an intravenous injection of methylprednisolone (16mg/kg body weight, n=10), minocycline (45mg/kg body weight, n=10) or saline (n=11). Mean arterial blood pressure (MABP), intracranial pressure (ICP) and local cerebral blood flow (LCBF) over both hemispheres were recorded continuously for three hours following SAH. Neurological assessment was performed after 24 hours. Hippocampal damage was analyzed by immunohistochemical staining (caspase 3). RESULTS Treatment with methylprednisolone or minocycline did not result in a significant improvement of MABP, ICP or LCBF. Animals of both treatment groups showed a non-significant trend to better neurological recovery compared to animals of the control group. Mortality was reduced and hippocampal damage significantly attenuated in both methylprednisolone and minocycline treated animals. CONCLUSION The results of this study suggest that inflammatory processes may play an important role in the pathophysiology of EBI after SAH. Early treatment with the anti-inflammatory drugs methylprednisolone or minocycline in the acute phase of SAH has the potential to reduce brain damage and exert a neuroprotective effect.

中文翻译：

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早期抗炎治疗可减轻大鼠 Sah 后的脑损伤。

背景技术早期炎症过程可能在蛛网膜下腔出血（SAH）后早期脑损伤（EBI）的发展中发挥重要作用。实验研究表明，抗炎和膜稳定药物可能具有有益的作用，尽管其潜在机制尚不完全清楚。本研究的目的是探讨甲基强的松龙和米诺环素早期治疗对实验性 SAH 后脑灌注和 EBI 的影响。方法 使用血管内丝模型对雄性 Sprague-Dawley 大鼠进行 SAH。SAH后30分钟，他们被随机分配接受静脉注射甲泼尼龙（16mg/kg体重，n=10）、米诺环素（45mg/kg体重，n=10）或生理盐水（n=11）。SAH 后连续三个小时连续记录两个半球的平均动脉血压 (MABP)、颅内压 (ICP) 和局部脑血流量 (LCBF)。24小时后进行神经学评估。通过免疫组织化学染色（半胱天冬酶 3）分析海马损伤。结果 甲泼尼龙或米诺环素治疗并未导致 MABP、ICP 或 LCBF 显着改善。与对照组的动物相比，两个治疗组的动物均表现出不显着的神经功能恢复趋势。甲泼尼龙和米诺环素治疗的动物死亡率降低，海马损伤显着减轻。结论 本研究结果表明炎症过程可能在 SAH 后 EBI 的病理生理学中发挥重要作用。在 SAH 急性期早期使用抗炎药物甲基强的松龙或米诺环素治疗有可能减少脑损伤并发挥神经保护作用。