Gastric cancer is the fourth most common cancer worldwide and the third most common in Asia, with a high mortality. Photodynamic therapy (PDT) is a new treatment for cancer. With advantages of minimum invasiveness, small adverse side effects and high selectivity, PDT can be used as palliative treatment for patients with advanced gastric cancer. YLG I, also known as 2-(1 hexyloxyethyl)-2-devinyl porphin e6 trisodium salt (HCE6), is a recently developed photosensitizer. A previous study showed that HCE6 significantly inhibited the growth of QBC939 human cholangiocarcinoma cells. However, the effects and mechanisms of HCE6 on gastric cancer cell suppression are not known. In this study, we investigated the effects of HCE6 on the human gastric cancer cell line MKN45 and found that at the concentration of 2.0 mg/L, HCE6 almost completely killed MKN45 cells at a light intensity of 3.6 J/cm². RNAseq results confirmed that mitochondria and endoplasmic reticulum (ER)-mediated apoptosis was involved in the effects of HCE6 on cell death, and we also found that HCE6 induced chromosome conformational changes in the early phase of apoptosis. The results of our study help elucidate the molecular mechanisms underlying HCE6-mediated inhibition of gastric cancer cell growth and provide a theoretical basis and molecular targets for the treatment of gastric cancer.

中文翻译：

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新型基于卟啉的HCE6光敏剂对MKN45人胃癌细胞活性的抑制作用及其分子机理。

胃癌是全球第四大常见癌症，在亚洲第三大常见癌症，死亡率很高。光动力疗法（PDT）是一种针对癌症的新疗法。PDT具有微创，微弱的副作用和高选择性的优势，可以作为晚期胃癌患者的姑息治疗。YLG I，也称为2-（1己氧基氧基乙基）-2-癸基卟啉e6三钠盐（HCE6），是最近开发的光敏剂。先前的研究表明，HCE6显着抑制QBC939人胆管癌细胞的生长。然而，HCE6对胃癌细胞抑制的作用和机制尚不清楚。在这项研究中，我们研究了HCE6对人胃癌细胞MKN45的影响，发现浓度为2.0 mg / L时，HCE6以3.6 J /cm²的光强度几乎完全杀死了MKN45细胞。RNAseq结果证实线粒体和内质网（ER）介导的凋亡参与了HCE6对细胞死亡的影响，我们还发现HCE6诱导了凋亡早期的染色体构象变化。我们的研究结果有助于阐明HCE6介导的抑制胃癌细胞生长的分子机制，并为治疗胃癌提供理论依据和分子靶标。