The prion infection is a conversion of host encoded prion protein (PrP) from its cellular isoform PrP(C) into the pathological and infectious isoform PrP(Sc); the conversion process was investigated by in vitro studies using recombinant and cellular PrP and natural PrP(Sc). We present a brief summary of the results determined with our in vitro conversion system and the derived mechanistic models. We describe well characterized intermediates and precursor states during the conversion process, kinetic studies of spontaneous and seeded fibrillogenesis and the impact of the membrane environment.

中文翻译：

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朊病毒感染：种子纤维化或更多？

朊病毒感染是宿主编码的朊病毒蛋白 (PrP) 从其细胞同种型 PrP(C) 转化为病理性和感染性同种型 PrP(Sc)；通过使用重组和细胞 PrP 以及天然 PrP(Sc) 的体外研究，对转化过程进行了调查。我们简要总结了用我们的体外转换系统和派生的机械模型确定的结果。我们描述了转化过程中表征良好的中间体和前体状态、自发和种子原纤维发生的动力学研究以及膜环境的影响。