Here, we have reported that keratinocytes respond to the microflow‐induced shear stress both at the collective and individual cell level. Using a microfluidic setup, we categorically showed that low shear stress of magnitude 0.06 dyne/cm² could induce morphological variation and cytoskeletal reorganization in keratinocyte, whereas higher shear stress (6 dyne/cm²) resulted in cellular disruption. Using a series of blocker molecules specific to different mechanotransducers, we demonstrated the pivotal role of actin network in keratinocyte mechanoresponsiveness in conjugation with myosin and lipid rafts. Flow‐induced shear stress also induced significant elevation in E‐cadherin and Zonula occludens‐1 (ZO‐1) expression levels. We further showed that under the influence of shear stress, the extent of colocalization of E‐cadherin and ZO‐1 was more at the cell–cell junction that indicates an improvement in the epithelial phenotype. An increase in the expression of nuclear lamin was also observed in the sheared cells that suggest the transmission of mechanical signals to the nucleus. It is envisioned that this study may find its application in basic and applied organogenesis of the epidermis.

中文翻译：

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角质形成细胞对微流诱导的剪切应力具有机械响应。

在这里，我们报道了角质形成细胞在集体细胞水平和个体细胞水平上均对微流诱导的切应力作出反应。使用微流控装置，我们明确表明，0.06达因/厘米^2的低剪切应力可以诱导角质形成细胞的形态变化和细胞骨架重组，而较高的剪切应力（6达因/ cm ^2））导致细胞破裂。使用一系列针对不同机械换能器的阻滞剂分子，我们证明了肌动蛋白网络在与肌球蛋白和脂质筏结合中在角质形成细胞机械反应中的关键作用。流动引起的剪切应力也导致E-钙粘蛋白和Zonula occludens-1（ZO-1）表达水平显着升高。我们进一步表明，在剪切应力的影响下，E-cadherin和ZO-1的共定位程度在细胞间连接处更多，这表明上皮表型有所改善。在剪切细胞中还观察到核纤层蛋白表达的增加，表明机械信号传递到核。可以预见，该研究可以发现其在表皮的基本和应用器官发生中的应用。