Since the report of the Edmonton protocol in 2000, islet transplantation has been implemented worldwide, and xenotransplantation using porcine islets has also been reported. In addition, many basic experiments using pancreatic islets and exocrine tissue after isolation have been reported. Recently, exocrine cells have been found to be essential for inducing the differentiation of pancreatic islets. Therefore, the importance of the culture conditions for pancreatic tissue when conducting experiments using pancreatic tissue is also increasing. In this study, we focused on the coat material and examined the adhesive properties of porcine pancreatic islets and exocrine tissue after isolation. Porcine islet isolation was performed, and isolated islets (purity ≥95%) and exocrine tissue (purity ≥99%) were used to achieve adhesion to several extracellular matrixes, fibronectin, collagen type I, collagen type IV, laminin I, fibrinogen, and bovine serum albumin (BSA). DMEM with 0.5% FBS was used as the assay medium. For exocrine tissue, the adhesion was promoted in fibronectin, collagen type I, laminin I, and fibrinogen. The adhesive ability to fibronectin was more than twice that to BSA, while the adhesive ability to collagen type I, laminin I, and fibrinogen was less than twice that to BSA. For islets, the adhesive ability to fibronectin was weaker than that of exocrine tissue. Furthermore, the adhesion effect in fibronectin was obtained within 30 minutes and in medium containing little serum for both islets and exocrine tissues. These data suggest that fibronectin may be useful for the adhesion of pancreatic tissue.

自2000年埃德蒙顿协议的报告以来，胰岛移植已在世界范围内实施，并且还报道了使用猪胰岛的异种移植。另外，已经报道了使用胰岛和分离后的外分泌组织的许多基础实验。最近，已发现外分泌细胞对于诱导胰岛的分化是必不可少的。因此，当使用胰腺组织进行实验时，培养条件对胰腺组织的重要性也在增加。在这项研究中，我们着眼于涂层材料，并研究了分离后的猪胰岛和外分泌组织的粘附特性。进行猪胰岛分离，以及分离的胰岛（纯度≥95％）和外分泌组织（纯度≥99％）用于粘附于几种细胞外基质，纤连蛋白，I型胶原，IV型胶原，层粘连蛋白I，纤维蛋白原和牛血清白蛋白（BSA） 。具有0.5％FBS的DMEM用作测定培养基。对于外分泌组织，在纤连蛋白，I型胶原，层粘连蛋白I和纤维蛋白原中促进了粘附。对纤连蛋白的粘附能力是对BSA的两倍以上，而对I型胶原，层粘连蛋白I和纤维蛋白原的粘附能力却比对BSA的粘附能力小两倍。对于胰岛，对纤连蛋白的粘附能力弱于外分泌组织。此外，在纤连蛋白中和在胰岛和外分泌组织的血清都很少的培养基中，在30分钟内即可获得粘附效果。