In bioenergetic research Paracoccus denitrificans has been used as an interesting model to elucidate the mechanisms of bacterial energy transduction. Genes for protein complexes of the respiratory chain and for proteins which are involved in periplasmic electron transport have been cloned and sequenced. Conjugational gene transfer has allowed the construction of site-specific mutant strains. Complementation experiments did not only open the field for site-directed mutagenesis and investigation of the structure/function relationship of the various electron-transport proteins, but also allowed first insights into processes like oxygen-dependent gene regulation or the assembly of electron-transport complexes. Also data will be presented that characterize two restriction-/modification systems, the codon usage and the promoter sequences of Paracoccus. Details will be given about the extrachromosomal localization of a duplicated cytochrome oxidase subunit I gene on one of the Paracoccus megaplasmids.

中文翻译：

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反硝化副球菌的遗传学。

在生物能研究中，反硝化副球菌已被用作阐明细菌能量转导机制的有趣模型。已经克隆并测序了呼吸链蛋白复合物的基因以及参与周质电子运输的蛋白的基因。共轭基因转移已经允许构建位点特异性突变株。补充实验不仅为定点诱变和研究各种电子传输蛋白的结构/功能关系开辟了领域，而且还使人们首次了解了氧依赖性基因调控或电子传输复合物组装等过程。还将提供表征两个限制/修饰系统的数据，所述密码子使用和副球菌的启动子序列。