The mosquitoes studied were Culex pipiens quinquefasciatus Say, C. tarsalis Coquillett, Aedes nigromaculis (Ludlow), and A. taeniorhynchus (Wiedemann). Oviposition attractants for each species were extracted with diethyl ether from the distillate of the water in which each species was breeding or supposed to breed. Two milliliters of the extracted concentrated attractants were prepared for each species from 100 or 120 liters of the breeding waters. Bioassays of oviposition attractants, from 4 distinct types of breeding sources, with the 4 species of mosquitoes were run in the laboratory. Oviposition attractants for C. tarsalis, A. nigromaculis , and A. taeniorhynchus were found, for the first time, to exist in their natural breeding waters. C. tarsalis oviposition attractants were 4.3 times as attractive as distilled water to this species at dosages of 50 and 5 μliters of the concentrate. A. nigromaculis attractants were 4.3 and 8.5 times as attractive as distilled water at dosages of 50 and 5 µliters, respectively. A. taeniorhynchus attractants stimulated 100% oviposition by this species at a 50-µliter dosage as compared with distilled water. The attractants for C. p. quinquefasciatus yielded attractiveness ratios of 4.7 and 2.8 at 50 and 5 µliters, respectively. Oviposition attractants for C. tarsalis, A. nigromaculis , and A. taeniorhynchus were, however, not markedly attractive to the other species of mosquitoes and showed repellency in some cases. There was an exception to this observation in that A. nigromaculis attractants were attractive to C. tarsalis at the 5-µliter dosage. C. p. quinquefasciatus was attracted to the attractants of all 4 species and was found to be nonspecific in its response to the various oviposition attractants. Based on the worldwide distribution of this species, this broad range of response is to be expected.

中文翻译：

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天然繁殖水域4种蚊子1的产卵引诱剂

研究的蚊子是 Culex pipiens quinquefasciatus Say、C. tarsalis Coquillett、Aedes nigromaculis (Ludlow) 和 A. taeniorhynchus (Wiedemann)。每个物种的产卵引诱剂是用乙醚从每个物种繁殖或应该繁殖的水中的蒸馏物中提取的。从 100 或 120 升的繁殖水中为每个物种制备 2 毫升提取的浓缩引诱剂。在实验室中对来自 4 种不同类型的繁殖来源的产卵引诱剂和 4 种蚊子进行了生物测定。首次发现 C. tarsalis、A. nigromaculis 和 A. taeniorhynchus 的产卵引诱剂存在于它们的天然繁殖水域中。C. tarsalis 产卵引诱剂为 4。在 50 和 5 微升浓缩液的剂量下，对该物种的吸引力是蒸馏水的 3 倍。在剂量为 50 和 5 微升时，黑斑曲霉引诱剂的吸引力分别是蒸馏水的 4.3 和 8.5 倍。与蒸馏水相比，A. taeniorhynchus 引诱剂以 50 微升的剂量刺激了该物种 100% 的产卵。C. p. 的引诱剂 quinquefasciatus 在 50 和 5 微升时的吸引力比分别为 4.7 和 2.8。然而，C. tarsalis、A. nigromaculis 和 A. taeniorhynchus 的产卵引诱剂对其他种类的蚊子没有明显的吸引力，并且在某些情况下显示出驱虫性。这一观察结果有一个例外，即黑斑曲霉引诱剂在 5 微升剂量下对跗骨曲霉具有吸引力。C.p. quinquefasciatus 被所有 4 种物种的引诱剂所吸引，并且发现它对各种产卵引诱剂的反应是非特异性的。根据该物种的全球分布，预计会有如此广泛的反应。