BACKGROUND BMP signaling is involved in myriad metazoan developmental processes, and study of this pathway in Drosophila has contributed greatly to our understanding of its molecular and genetic mechanisms. These studies have benefited not only from Drosophila's advanced genetic tools, but from complimentary in vitro culture systems. However, the commonly-used S2 cell line is not intrinsically sensitive to the major BMP ligand Dpp and must therefore be augmented with exogenous pathway components for most experiments. RESULTS Herein we identify and characterize the responses of Drosophila ML-DmD17-c3 cells, which are sensitive to Dpp stimulation and exhibit characteristic regulation of BMP target genes including Dad and brk. Dpp signaling in ML-DmD17-c3 cells is primarily mediated by the receptors Put and Tkv, with additional contributions from Wit and Sax. Furthermore, we report complex regulatory feedback on core pathway genes in this system. CONCLUSIONS Native ML-DmD17-c3 cells exhibit robust transcriptional responses to BMP pathway induction. We propose that ML-DmD17-c3 cells are well-suited for future BMP pathway analyses.

中文翻译：

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果蝇 ML-DmD17-c3 细胞对 Dpp 反应强烈，并表现出对 BMP 信号成分的复杂转录反馈。

背景 BMP 信号参与无数后生动物的发育过程，在果蝇中研究该途径对我们了解其分子和遗传机制做出了巨大贡献。这些研究不仅受益于果蝇的先进遗传工具，而且受益于互补的体外培养系统。然而，常用的 S2 细胞系对主要 BMP 配体 Dpp 本质上并不敏感，因此必须在大多数实验中增加外源途径成分。结果 在此我们鉴定并表征了果蝇 ML-DmD17-c3 细胞的反应，这些细胞对 Dpp 刺激敏感并且表现出对包括 Dad 和 brk 在内的 BMP 靶基因的特征调节。ML-DmD17-c3 细胞中的 Dpp 信号传导主要由受体 Put 和 Tkv 介导，还有来自 Wit 和 Sax 的额外贡献。此外，我们报告了该系统中核心通路基因的复杂调节反馈。结论 天然 ML-DmD17-c3 细胞对 BMP 通路诱导表现出强烈的转录反应。我们建议 ML-DmD17-c3 细胞非常适合未来的 BMP 通路分析。