The bacterial species Escherichia coli has proven to be a powerful tool in the molecular analysis of polyhydroxyalkanoate (PHA) biosynthesis. In addition, E. coli holds promise as a source for economical PHA production. Using this microorganism, clones have been developed in our laboratory which direct the synthesis of poly-beta-hydroxybutyrate (PHB) to levels as high as 95% of the cell dry weight. These clones have been further enhanced by the addition of a genetically mediated lysis system that allows the PHB granules to be released gently and efficiently. This paper describes these developments, as well as the use of an E. coli strain to produce the copolymer poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-co-3HV).

中文翻译：

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在重组大肠杆菌中生产多羟基链烷酸酯。

在多羟基链烷酸酯（PHA）生物合成的分子分析中，大肠杆菌已被证明是一种强大的工具。此外，大肠杆菌有望成为经济生产PHA的来源。使用这种微生物，在我们的实验室中已经开发出克隆，这些克隆将聚-β-羟基丁酸酯（PHB）的合成水平提高到细胞干重的95％。这些克隆通过添加遗传介导的裂解系统得到了进一步增强，该裂解系统可使PHB颗粒缓慢有效地释放。本文描述了这些进展，以及使用大肠杆菌菌株生产共聚物聚-（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHB-co-3HV）。