Optimization and standardization of immunohistochemistry (IHC) protocols within and between laboratories requires reproducible positive and negative control samples. In many situations, suitable tissue or cell line controls are not available. We demonstrate here a method to incorporate target antigens into synthetic protein gels that can serve as IHC controls. The method can use peptides, protein domains, or whole proteins as antigens, and is compatible with a variety of fixation protocols. The resulting gels can be used to create tissue microarrays (TMAs) with a range of antigen concentrations that can be used to objectively quantify and calibrate chromogenic, fluorescent, or mass spectrometry-based IHC protocols. The method offers an opportunity to objectively quantify IHC staining results, and to optimize and standardize IHC protocols within and between laboratories. (J Histochem Cytochem 58:XXX-XXX, 2019).

中文翻译：

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合成抗原凝胶作为标准化和定量免疫组织化学的实用对照。

实验室内部和实验室之间免疫组织化学（IHC）方案的优化和标准化需要可重复的阳性和阴性对照样品。在许多情况下，没有合适的组织或细胞系对照。我们在这里展示了一种将靶抗原掺入可用作IHC对照的合成蛋白凝胶的方法。该方法可以使用肽，蛋白质结构域或完整蛋白质作为抗原，并且与多种固定方案兼容。所得的凝胶可用于创建具有一定范围抗原浓度的组织微阵列（TMA），可用于客观地定量和校准基于发色，荧光或质谱的IHC方案。该方法提供了客观量化IHC染色结果的机会，并优化和标准化实验室内部和实验室之间的IHC协议。（J Histochem Cytochem 58：XXX-XXX，2019）。