The phylogenetic composition and physiological profiles of bacterial communities in freshwater springs were evaluated during the blooming and non-blooming stages of the invasive plant species, Hydrilla verticillata. Community-level physiological profiles (CLPPs) and pyrosequencing of 16S rRNA gene amplicons were used to study potential Hydrilla mediated shifts in the physiological potential and phylogenetic composition of the bacterial community in infested systems. The results of CLPP revealed that the microbes in the Hydrilla invaded sites utilized less substrates during blooming periods than during non-blooming periods of the plant. Spearman’s rank correlation analysis showed some relationships between the relative abundances of bacterial taxa and the Biolog substrate utilization pattern. The relative abundance of the identified taxa showed some striking differences based on the blooming status of Hydrilla and to a lesser extent on site variation. The relative abundance of Actinobacteria, Bacteriodetes, and Verrucomicrobia was generally higher during Hydrilla blooms, while Deltaproteobacteria was generally higher during non-blooming stages of Hydrilla. The detected genera also varied based on the blooming stages of the plant. Based on the findings, it appears that Hydrilla alters the phylogenetic composition and structure of the bacterial community during the blooming stage.

中文翻译：

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入侵植物轮枝藻侵染的淡水泉水细菌群落结构

在入侵植物 Hydrilla verticillata 的开花和非开花阶段评估了淡水泉中细菌群落的系统发育组成和生理特征。社区级生理特征 (CLPP) 和 16S rRNA 基因扩增子的焦磷酸测序用于研究黑藻介导的受感染系统中细菌群落生理潜力和系统发育组成的潜在变化。CLPP 的结果表明，黑藻入侵位点的微生物在开花期间比在非开花期间利用更少的底物。Spearman 等级相关分析显示细菌分类群的相对丰度与 Biolog 底物利用模式之间存在一些关系。已识别类群的相对丰度显示出一些显着差异基于黑藻的开花状态和在较小程度上的现场变化。在黑藻开花期间，放线菌、拟杆菌和疣微菌的相对丰度通常较高，而在黑藻非开花阶段，Deltaproteobacteria 的相对丰度通常较高。检测到的属也因植物的开花阶段而异。根据这些发现，Hydrilla 似乎在开花阶段改变了细菌群落的系统发育组成和结构。而 Deltaproteobacteria 在黑藻非开花阶段普遍较高。检测到的属也因植物的开花阶段而异。根据这些发现，Hydrilla 似乎在开花阶段改变了细菌群落的系统发育组成和结构。而 Deltaproteobacteria 在黑藻非开花阶段普遍较高。检测到的属也因植物的开花阶段而异。根据这些发现，Hydrilla 似乎在开花阶段改变了细菌群落的系统发育组成和结构。