Abstract We investigated effects of apterous mutation ap 56f on circadian locomotor activity, eclosion rhythms, and transcript levels of period and timeless in Drosophila. We investigated circadian locomotor activity and eclosion rhythms in ap 56f and wild-type flies, their F1 and F2 offspring, and wingless vestigial mutants and show that ap 56f disrupts circadian locomotor rhythms in a genetically recessive manner, that is not caused by the absence of wings. The apblt strain also showed impaired circadian activity rhythms, providing independent evidence for a significant role of apterous in circadian locomotor rhythm expression. The ap 56f mutation did not disrupt a circadian eclosion rhythm or rhythmic expression of the period and timeless clock genes, indicating that apterous is not essential for circadian clock function, but is necessary for coupling locomotor activity to a circadian clock. Timeless transcription was reduced in ap 56f flies in 12:12 LD, suggesting that apterous may modulate core clock gene expression.

中文翻译：

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果蝇 apterous56f 突变损害昼夜运动活动

摘要 我们研究了 ap 56f 突变对果蝇昼夜节律运动活动、羽化节律以及周期和时间转录水平的影响。我们调查了 ap 56f 和野生型果蝇、它们的 F1 和 F2 后代以及无翅退化突变体的昼夜运动活动和羽化节律，并表明 ap 56f 以遗传隐性方式破坏昼夜运动节律，这不是由于缺乏翅膀。apblt 菌株还显示昼夜节律活动节律受损，为 apterous 在昼夜节律运动节律表达中的重要作用提供了独立证据。ap 56f 突变没有破坏昼夜节律羽化节律或周期和永恒时钟基因的节律表达，表明 apterous 对生物钟功能不是必需的，但对于将运动活动耦合到生物钟是必要的。在 12:12 LD 中，ap 56f 果蝇的永恒转录减少，表明 apterous 可能调节核心时钟基因表达。