Efficient utilization of lignocellulosic Miscanthus biomass for the production of biochemicals, such as ethanol, is challenging due to its recalcitrance, which is influenced by the individual plant cell wall polymers and their interactions. Lignocellulosic biomass composition differs depending on several factors, such as plant age, harvest date, organ type, and genotype. Here, four selected Miscanthus genotypes (Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus × giganteus, Miscanthus sinensis × Miscanthus sacchariflorus hybrid) were grown and harvested, separated into stems and leaves, and characterized for their non‐starch polysaccharide composition and structures, lignin contents and structures, and hydroxycinnamate profiles (monomers and ferulic acid dehydrodimers). Polysaccharides of all genotypes are mainly composed of cellulose and low‐substituted arabinoxylans. Ratios of hemicelluloses to cellulose were comparable, with the exception of Miscanthus sinensis that showed a higher hemicellulose/cellulose ratio. Lignin contents of Miscanthus stems were higher than those of Miscanthus leaves. Considering the same organs, the four genotypes did not differ in their Klason lignin contents, but Miscanthus × giganteus showed the highest acetylbromide soluble lignin content. Lignin polymers isolated from stems varied in their S/G ratios and linkage type distributions across genotypes. p‐Coumaric acid was the most abundant ester‐bound hydroxycinnamte monomer in all samples. Ferulic acid dehydrodimers were analyzed as cell wall cross‐links, with 8‐5‐coupled diferulic acid being the main dimer, followed by 8‐O‐4‐, and 5‐5‐diferulic acid. Contents of p‐coumaric acid, ferulic acid, and ferulic acid dimers varied depending on genotype and organ type. The largest amount of cell wall cross‐links was analyzed for Miscanthus sinensis.

中文翻译：

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芒草细胞壁聚合物的表征。

有效利用木质纤维素芒草生物质生产生物化学品（例如乙醇）具有挑战性，因为它的顽抗性受到单个植物细胞壁聚合物及其相互作用的影响。木质纤维素生物质组成因多种因素而异，例如植物年龄、收获日期、器官类型和基因型。在这里，种植和收获了四种选定的芒草基因型（芒草、芒草、芒草、芒草× 芒草 杂种），分离茎和叶，并对其非淀粉多糖组成和结构、木质素含量和木质素含量进行了 表征 。结构和羟基肉桂酸酯概况（单体和阿魏酸脱氢二聚体）。所有基因型的多糖主要由纤维素和低取代阿拉伯木聚糖组成。半纤维素与纤维素的比率相当，但芒草除外，其显示出较高的半纤维素/纤维素比率。芒草茎的木质素含量高于芒草叶。考虑到相同的器官，四种基因型的 Klason 木质素含量没有差异，但芒草的 乙酰 溴可溶性木质素含量最高。从茎中分离出的木质素聚合物的 S/G 比率和不同基因型的连接类型分布各不相同。对香豆酸是所有样品中最丰富的酯键羟基肉桂酸酯单体。阿魏酸脱氢二聚体作为细胞壁交联进行分析，其中 8-5-偶联二阿魏酸是主要二聚体，其次是 8-O-4- 和 5-5-二阿魏酸。对香豆酸、阿魏酸和阿魏酸二聚体的含量根据基因型和器官类型而变化。对芒草的细胞壁交联量进行了分析。