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A method for generating virus-free cassava plants to combat viral disease epidemics in Africa
Physiological and Molecular Plant Pathology ( IF 2.8 ) Pub Date : 2019-01-01 , DOI: 10.1016/j.pmpp.2018.09.002
M N Maruthi 1 , E Charles Whitfield 1 , Gerald Otti 1 , Silver Tumwegamire 2 , Edward Kanju 2 , James P Legg 2 , Geoffrey Mkamilo 3 , Robert Kawuki 4 , Ibrahim Benesi 5 , Anabela Zacarias 6 , Therezia Munga 7 , Francis Mwatuni 8 , Edward Mbugua 9
Affiliation  

Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture, chemotherapy and thermotherapy. In the first cycle of our virus-indexing procedure, we successfully cleaned 27 of the 31 varieties (87%), and after an additional three cleaning cycles, all plants were virus-free. Virus-free tissue-cultured plants were shipped back to Africa for distribution to farmers. This first cross-boundary effort provides important lessons for mitigating the two-major cassava viral diseases.

中文翻译:

一种产生脱病毒木薯植物以对抗非洲病毒性疾病流行的方法

在这里,我们报告了一种清除木薯植物免受导致非洲木薯花叶病和褐条病的病毒感染的方法。来自马拉维、莫桑比克、肯尼亚、坦桑尼亚和乌干达的抗性或耐受品种的受感染植物在英国通过组织培养、化疗和热疗的组合进行了清理。在病毒索引程序的第一个周期中,我们成功清洁了 31 个品种中的 27 个品种 (87%),经过另外三个清洁周期后,所有植物都不含病毒。无病毒的组织培养植物被运回非洲分发给农民。这项首次跨界努力为减轻两种主要木薯病毒病提供了重要的经验教训。
更新日期:2019-01-01
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