The localization of Cassava brown streak virus (CBSV) in cassava (Manihot esculenta) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed precipitation in CBSV-infected but not CBSV-free tissues, demonstrating successful localization of CBSV. Microscopic inspection showed significantly larger (P < 0.001) midribs in CBSV-infected compared with control (uninfected) leaves. Viral accumulation occurred in middle and lower but rarely in young upper leaves. This immunohistochemical method for virus localization will be invaluable for efficient screening of CBSV and for breeding resistant cassava.

中文翻译：

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木薯褐条病毒的免疫组织化学定位及其对木薯叶形态的影响


确定了木薯褐条病毒 (CBSV) 在木薯 (Manihot esculenta) 叶组织中的定位，并评估了 CBSV 感染组织中的细胞形态变化。使用逆转录酶聚合酶链式反应，用 CBSV 特异性引物筛选有 CBSV 症状的叶子。免疫组织化学反应显示在 CBSV 感染的组织中出现沉淀，但在无 CBSV 的组织中没有出现沉淀，这表明 CBSV 的成功定位。显微镜检查显示，与对照（未感染）叶子相比，CBSV 感染的叶子中脉明显更大（P < 0.001）。病毒积累发生在中下部叶，但很少发生在幼嫩的上部叶中。这种用于病毒定位的免疫组织化学方法对于有效筛选 CBSV 和育种抗性木薯具有不可估量的价值。