In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study, we evaluated next-generation sequencing (NGS) for virus detection following yam propagation using a robust tissue culture methodology. We detected and assembled the genomes of novel isolates of already characterised viral species of the genera Badnavirus and Potyvirus, confirming the utility of NGS in diagnosing yam viruses and contributing towards the safe distribution of germplasm.

中文翻译：

---

组织培养和下一代测序：检测山药（薯蓣属）病毒的组合方法

体外培养为山药种质保存、繁殖和国际分销提供了许多优势。然而，生成的组织中病毒滴度较低，对可靠的病毒检测提出了挑战，这使得很难确保种植材料不含病毒。在这项研究中，我们使用稳健的组织培养方法评估了用于山药繁殖后病毒检测的下一代测序（NGS）。我们检测并组装了巴德纳病毒属和马铃薯蒂病毒属已鉴定病毒种的新分离株的基因组，证实了 NGS 在诊断山药病毒方面的实用性，并有助于种质的安全分布。