Usually, mapping studies in potato are performed with segregating populations from crosses between highly heterozygous diploid or tetraploid parents. These studies are hampered by a high level of genetic background noise due to the numerous segregating alleles, with a maximum of eight per locus. In the present study, we aimed to increase the mapping efficiency by using progenies from diploid inbred populations in which at most two alleles segregate. Selfed progenies were generated from a cross between S. tuberosum (D2; a highly heterozygous diploid) and S. chacoense (DS; a homozygous diploid clone) containing the self-incompatibility overcoming S locus inhibitor (Sli-gene). The Sli-gene enables self-pollination and the generation of selfed progenies. One F2 population was used to map several quality traits, such as tuber shape, flesh and skin color. Quantitative trait loci were identified for almost all traits under investigation. The identified loci partially coincided with known mapped loci and partially identified new loci. Nine F3 populations were used to validate the QTLs and monitor the overall increase in the homozygosity level.

中文翻译：

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利用 S. tuberosum × S. chacoense 自交后代在二倍体马铃薯中进行 QTL 定位

通常，马铃薯的作图研究是通过从高度杂合的二倍体或四倍体亲本之间的杂交中分离种群来进行的。由于存在大量分离的等位基因，每个基因座最多有 8 个等位基因，这些研究受到高水平遗传背景噪音的阻碍。在本研究中，我们旨在通过使用最多两个等位基因分离的二倍体近交种群的后代来提高作图效率。自交后代是从马铃薯（D2；高度杂合二倍体）和沙科马铃薯（DS；纯合二倍体克隆）之间的杂交产生的，其中包含克服 S 基因座抑制剂（Sli 基因）的自交不亲和性。Sli 基因能够实现自花授粉和自交后代的产生。一个 F2 种群被用来绘制几个质量性状，如块茎形状、肉和皮肤的颜色。几乎所有调查的性状都确定了数量性状基因座。确定的基因座与已知的映射基因座部分重合，部分确定了新基因座。九个 F3 群体用于验证 QTL 并监测纯合度水平的总体增加。