Glycosylation is a widespread post-translational modification that plays important roles in health and disease. As glycan sequence and structure are not directly coded into the genome, our understanding of glycans and their functions in biological systems is much more primitive than that of DNA and proteins.Recently, printed glycan microarrays (glycoarrays) have emerged as powerful, high-throughput tools for screening glycan-protein interactions[1,2], and have been applied in disease detection [3], drug discovery [4], the study of immunity [5], and host-pathogen interactions [1, 2], among others.Unfortunately, glycoarray applications are currently limited by the expensive and complex methods available to synthesize glycans or alternatively, by the challenges in identifying and tagging glycans from natural sources [6, 7]. In this issue of Biotechnology Journal, Çelik et al. [8] introduce a potentially powerful new method for facile, scalable production, and purification of glycans compatible with microarray patterning. Çelik et al.’s [8] approach is based on innovative deployment of filamentous phage display so that the displayed proteins can be tagged with specific glycans of interest (glycophages) and subsequently patterned in array format.

中文翻译：

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引入噬菌体阵列：噬菌体的简便生产，纯化和模式化。

糖基化是广泛的翻译后修饰，其在健康和疾病中起重要作用。由于聚糖的序列和结构没有直接编码到基因组中，因此我们对聚糖及其在生物系统中的功能的了解远比DNA和蛋白质更原始。最近，印刷的聚糖微阵列（glycoarrays）出现了功能强大，高通量的特点用于筛选糖蛋白相互作用的工具[1,2]，并已用于疾病检测[3]，药物发现[4]，免疫研究[5]和宿主-病原体相互作用[1、2]不幸的是，糖阵列的应用目前受到可用于合成聚糖的昂贵和复杂方法的限制，或者受制于从天然来源鉴定和标记聚糖的挑战[6，7]。在本期《生物技术杂志》中，Çelik等人。[8]引入了一种潜在的功能强大的新方法，用于与微阵列模式兼容的聚糖的简便，可扩展的生产和纯化。Çelik等人的方法[8]基于丝状噬菌体展示的创新部署，因此可以用感兴趣的特定聚糖（噬菌体）标记展示的蛋白质，然后以阵列形式进行构图。