Paenibacillus alvei NP75, a Gram-positive bacterium, produces two different antimicrobial peptides, paenibacillin N and P, which has potent antimicrobial activity against many clinical pathogens. The synthesis pattern of these antimicrobial peptides by P. alvei NP75 was studied extensively. The results were outstanding in a way that the paenibacillin N was synthesized irrespective of the growth of bacteria (non-ribosomal mediated), whereas paenibacillin P production was carried out by ribosomal mediated. In addition to the antimicrobial peptides, P. alvei NP75 also produces an immunogenic extracellular protease to defend itself from its own antimicrobial peptide, paenibacillin P. Furthermore, this immunogenic protease production was impaired by the addition of protease inhibitor, phenylmethylsulfonyl fluoride (PMSF). The sodium dodecyl sulfate (SDS) treated strain (mutant) failed to produce paenibacillin P, whereas the production of neither paenibacillin N nor the protease was affected by the plasmid curing. The plasmid curing studies that divulge the genes responsible for the synthesis of paenibacillin N and protease were found to be genome encoded, and paenibacillin P was plasmid encoded. We are reporting, first of its kind, the co-production of two different antimicrobial peptides from P. alvei NP75 through non-ribosomal and ribosomal pathways that could be used as effective antibiotics.

中文翻译：

---

肺泡杆菌NP75的两种抗菌肽的合成机理评估。

革兰氏阳性细菌肺泡杆菌NP75产生两种不同的抗菌肽，即Paenibacillin N和P，它们对许多临床病原体均具有有效的抗菌活性。肺炎链球菌NP75对这些抗菌肽的合成模式进行了广泛的研究。结果是杰出的，无论细菌的生长如何（非核糖体介导的），都能合成Paenibacillin N，而Paesebacillin P的生产是通过核糖体介导的。除抗菌肽外，P。alveiNP75还产生一种免疫原性的细胞外蛋白酶，以防御自身的抗菌肽paenibacillinP。此外，这种免疫原性蛋白酶的生产还受到蛋白酶抑制剂苯甲基磺酰氟（PMSF）的加入的影响。经十二烷基硫酸钠（SDS）处理的菌株（突变体）未能产生paenibacillin P，而质粒分离对paenibacillin N和蛋白酶的产生均无影响。发现泄露了负责合成paenibacillin N和蛋白酶的基因的质粒固化研究是基因组编码的，paenibacillin P是质粒编码的。我们首次报道了来自肺炎单胞菌的两种不同抗菌肽的共同生产 NP75可通过非核糖体和核糖体途径被用作有效的抗生素。