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Embedding cell monolayers to investigate nanoparticle-plasmalemma interactions at transmission electron microscopy.
European Journal of Histochemistry ( IF 2.1 ) Pub Date : 2019-03-29 , DOI: 10.4081/ejh.2019.3026
Manuela Costanzo 1 , Manuela Malatesta
Affiliation  

Transmission electron microscopy is the technique of choice to visualize the spatial relationships between nanoconstructs and cells and especially to monitor the uptake process of nanomaterials. It is therefore crucial that the cell surface be preserved in its integrity, to obtain reliable ultrastructural evidence: the plasmalemma represents the biological barrier the nanomaterials have to cross, and the mode of membrane-nanoconstruct interaction is responsible for the intracellular fate of the nanomaterials. In this paper, we describe a simple and inexpensive method to process cell monolayers for ultrastructural morphology and immunocytochemistry, ensuring consistent preservation of the cell surface and of the occurring interactions with nanoparticles of different chemical composition.

中文翻译:

嵌入细胞单层以在透射电子显微镜下研究纳米粒子与血浆的相互作用。

透射电子显微镜是一种可视化纳米结构与细胞之间的空间关系,尤其是监测纳米材料吸收过程的技术。因此至关重要的是,必须保持细胞表面的完整性,以获得可靠的超微结构证据:质膜障碍代表了纳米材料必须穿越的生物屏障,而膜-纳米结构相互作用的模式决定了纳米材料的细胞内命运。在本文中,我们描述了一种简单且便宜的方法来处理用于超微结构形态学和免疫细胞化学的细胞单分子层,以确保细胞表面以及与不同化学组成的纳米粒子之间发生的相互作用的一致保存。
更新日期:2019-11-01
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