In this study, was evaluated the chemical composition of a fraction from Syngonanthus nitens extract and its antimicrobial potential unloaded (Fr3) and loaded (F9Fr3) into a nanoemulsion (F9) composed of cholesterol as the oil phase (10%), polyoxyethylene 20-cetyl ether and soy phosphatidylcholine (2:1) as surfactant (20%), and a solution of phosphate buffer (pH 7.4) plus chitosan polymer dispersion (0.25%) as the aqueous phase (70%) to use for VVC treatment. Phytochemical procedures showed that Fr3 is rich in luteolin, which is responsible for the antimicrobial activity. F9 development showed satisfactory parameters for use in the vulvovaginal candidiasis (VVC) treatment, as F9 demonstrated pseudoplastic, elastic behavior, and adhesive properties on vaginal mucosa. In addition, we observed improvement in antimicrobial potential of Fr3 on planktonic and biofilms after incorporation in F9. Fr3 and F9Fr3 showed satisfactory parameters related to toxic profiles in cell lines and in a model of acute toxicity by Artemia salina. The in vivo VVC assay showed that F9Fr3 was more active than unloaded Fr3 in VVC treatment. In conclusion, this work showed that use of a fraction rich in luteolin can be a used as an antimicrobial for treatment of vaginal infections and that use of nanostructured lipid systems was an important factor in the biological efficacy of Fr3, especially in treatment of acute VVC.

中文翻译：

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基于纳米乳剂系统的Syngonanthus nitens（Bong。）Ruhland馏分的阴道内递送，用于念珠菌性念珠菌病的治疗。

在这项研究中，评估了Sygonanthus nitens的馏分的化学组成提取物及其抗菌潜力被卸载（Fr3）并被装载（F9Fr3）到纳米乳液（F9）中，该乳液由胆固醇作为油相（10％），聚氧乙烯20-鲸蜡醚和大豆磷脂酰胆碱（2：1）作为表面活性剂（20％ ）和磷酸盐缓冲液（pH 7.4）加壳聚糖聚合物分散体（0.25％）作为水相（70％）的溶液，用于VVC处理。植物化学过程表明，Fr3富含木犀草素，这是抗菌活性的原因。F9的发展显示出令人满意的参数，可用于外阴念珠菌病（VVC）治疗，因为F9表现出对阴道粘膜的假塑性，弹性行为和粘附特性。此外，我们观察到Fr3掺入F9后对浮游生物膜和生物膜的抗菌潜力有所提高。卤虫卤虫。的体内VVC实验显示F9Fr3比在VVC治疗卸载FR3更加活跃。总之，这项工作表明，使用富含木犀草素的馏分可以作为抗菌剂用于治疗阴道感染，并且使用纳米结构脂质系统是Fr3生物学功效的重要因素，尤其是在治疗急性VVC中。