In clinical diagnosis and treatment, it is very important to distinguish cancer cells from normal cells. Nuclear-selective fluorescent probe that specifically target tumors can not only make the difference in assessing tumor margins during surgery and then facilitating accurate resection of the tumor, but also can provide crucial biomedical information of tumor progress if it can be used for long-term dynamic visualization of nucleus in cancer. Herein, a novel fluorescent probe 3 was designed and characterized to be of low-toxicity and water-solubility. The biological evaluation indicated that probe 3 prefers nucleic acids rather than accumulation in non-neuclear sites while superior to the commercial available agent DAPI (4',6-diamidino-2-phenylindole), in terms of its character of aggregation-induced emission (AIE), large Stokes shift (175 nm) and light stability. Further experiments demonstrated that probe 3 can not only differentiate SW480 and SW620 (cancer cells) from GES-1 (normal cells) with high contrast (dyed in nuclear of cancer cells and not in nuclear of normal cells), but also used for tracking cancer cell nuclear for long time. Furthermore, 3D reconstruction fluorescence imaging proved that probe 3 was able for identifying colorectal cancer tissues from para-carcinoma tissues by a strong contrast. Therefore, in precise surgery of colorectal cancer, probe 3 may be a promising-agent for guiding of intraoperation.

中文翻译：

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长期跟踪癌细胞核并通过聚集诱导的基于发射的荧光探针鉴定大肠癌。

在临床诊断和治疗中，区分癌细胞与正常细胞非常重要。专门针对肿瘤的核选择性荧光探针不仅可以在评估手术过程中的肿瘤边缘，然后促进精确切除肿瘤方面有所作为，而且如果可以用于长期动态研究，还可以提供重要的肿瘤进展生物医学信息可视化癌症中的核。在本文中，设计了新颖的荧光探针3，并表征为低毒性和水溶性。生物学评估表明，探针3从聚集诱导发射的特性来看，它更喜欢核酸而不是在非核中积累，而优于市售药物DAPI（4'，6-diamidino-2-phenylindole）（ AIE），较大的斯托克斯位移（175 nm）和光稳定性。进一步的实验表明，探针3不仅可以以高对比度（在癌细胞核而不是正常细胞核中染色）与GES-1（正常细胞）区分SW480和SW620（癌细胞），而且还可以用于追踪癌症细胞核很长一段时间。此外，3D重建荧光成像证明，探针3能够通过强烈的对比从癌旁组织中识别出结直肠癌组织。因此，在大肠癌的精密外科手术中，探针3可能是指导术中指导的有前途的药物。而且还用于长时间追踪癌细胞核。此外，3D重建荧光成像证明，探针3能够通过强烈的对比从癌旁组织中识别出结直肠癌组织。因此，在大肠癌的精密外科手术中，探针3可能是指导术中指导的有前途的药物。而且还用于长时间追踪癌细胞核。此外，3D重建荧光成像证明，探针3能够通过强烈的对比从癌旁组织中识别出结直肠癌组织。因此，在大肠癌的精密外科手术中，探针3可能是指导术中指导的有前途的药物。