Magnetic iron oxide nanoparticles (MIONPs) were placed in the spotlight lately due to their excellent biocompatibility and the possibility to tailor their magnetic properties making them useful in a plethora of bioapplications, including magnetic resonance imaging and targeted nanoplatforms for anticancer drugs delivery. The aim of the present study consisted in achieving a toxicological profile of the biocompatible colloidal suspension of iron oxide nanoparticles coated with a double layer of oleic acid (Fe₃O₄ @OA) obtained by combustion method by performing in vitro (on human keratinocytes-HaCaT and human and murine melanoma cells) and in ovo studies on chick chorioallantoic membrane (HET-CAM assay). The colloidal suspension obtained proved to be stable in phosphate buffer saline and the size of the nanoparticles were in the range of 30 nm, an optimum size for biomedical applications. Fe₃O₄ @OA colloidal suspension reduced viability of human keratinocytes only at concentrations higher than 25 μg/mL, whereas in the case of melanoma cells the effect was observed at lower doses (starting with 10 μg/mL). An interesting phenomena was detected at the highest concentration tested (50 μg/mL) to all cell lines, more precisely, a particular enucleation process associated only with Fe₃O₄ @OA colloidal suspension stimulation. The irritant potential data evaluated by HET-CAM assay indicated the following hierarchy: Fe₃O₄ < Fe₃O₄ @OA < OA. Our results provide relevant information regarding the mechanism of action of Fe₃O₄ @OA that needs elucidation by future in vitro and in vivo studies.

中文翻译：

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油酸双重包覆的Fe 3 O 3纳米颗粒作为抗黑素瘤化合物，具有体内和体外评估的复杂机制。

磁性氧化铁纳米颗粒（MIONPs）由于其出色的生物相容性以及可以调整其磁性的可能性而在最近成为人们关注的焦点，从而使其可用于多种生物应用，包括磁共振成像和用于抗癌药物递送的靶向纳米平台。本研究的目的在于通过在体外（在人角质形成细胞-HaCaT和人上）进行燃烧，获得通过燃烧法获得的涂有双层油酸（Fe 3 O 3 @OA）的氧化铁纳米颗粒的生物相容性胶体悬浮液的毒理学概况。和鼠黑色素瘤细胞）和卵内鸡绒膜尿囊膜的研究（HET-CAM测定）。所获得的胶体悬浮液在磷酸盐缓冲液中证明是稳定的，纳米粒子的尺寸在30 nm的范围内，这是生物医学应用的最佳尺寸。Fe₃O₄@OA胶体悬浮液仅在浓度大于25降低人角质形成细胞的存活力更高μ克/ mL，在以较低的剂量下观察到的作用的黑色素瘤细胞的情况下，而（10开始μ克/毫升）。在测试的最高浓度检测到一个有趣的现象（50 μg / mL），更确切地说，是仅与Fe₃O3@OA胶体悬浮液刺激相关的特定去核过程。通过HET-CAM测定评估的刺激性潜在数据表明以下等级：Fe₃O₄<Fe₃O₄@ OA <OA。我们的结果提供了有关Fe₃O₄@ OA作用机理的相关信息，需要通过未来的体外和体内研究加以阐明。