The possibility of composite nanofibers being able to regenerate bone is an attractive proposition. Adenosine, which occurs naturally in humans, has been shown to promote the osteogenic differentiation of mesenchymal stem cells (MSCs) and osteoprogenitor cells. In this study, electrospun nanofibers of poly(3-hydroxybutyrate-co-3-hydroxybutyrate) (PHBV) doped with adenosine were demonstrated to exhibit excellent capacity for bone regeneration, after optimization of the electrospinning process. The biomechanical properties, hydrophilicity, biocompatibility, cellular performance of the nanofibers and adenosine release profile from the composite nanofibers were evaluated. The osteogenic capacity of the composite nanofibers in vitro and in vivo was systematically studied. Electrospun adenosine/PHBV nanofibers demonstrated excellent tissue biocompatibility. In addition, adenosine-loaded/PHBV electrospun nanofibers exhibited substantial bone regeneration capacity in vitro and in critical-sized rabbit cranial defects in vivo, which was greater than that of bone marrow MSC (BMSC)-loaded/PHBV electrospun nanofibers. Additionally, BMSCs/PHBV electrospun nanofibers required culture with BMSCs for a period of time prior to surgery, whereas the adenosine/PHBV electrospun nanofibers could be implanted directly. To date, there is seldom no studies have evaluated the capability of bone regeneration of electrospun nanofibers doped with adenosine. Using a simple fabrication process and with a structure similar to that of natural extracellular matrix (ECM), electrospun adenosine/PHBV nanofibers exhibited excellent biocompatibility and osteogenic capacity. In addition, adenosine is inexpensive, straightforward to obtain and store and so holds huge practical potential in bone tissue engineering applications.

中文翻译：

---

腺苷负载电纺纳米纤维的制备及其在骨再生中的应用。

复合纳米纤维能够再生骨骼的可能性是一个有吸引力的主张。腺苷（天然存在于人类中）已显示出促进间充质干细胞（MSCs）和骨祖细胞的成骨分化。在这项研究中，聚电纺纳米纤维（3-羟基丁酸酯共-3-羟基丁酸酯）（PHBV）掺杂有腺苷被证明表现出对骨再生优异的容量，电纺丝工艺的优化后。评估了纳米纤维的生物力学性能，亲水性，生物相容性，细胞性能以及复合纤维中腺苷的释放曲线。复合纳米纤维在体外和体内的成骨能力被系统地研究了。电纺腺苷/ PHBV纳米纤维具有出色的组织生物相容性。此外，载有腺苷/ PHBV的电纺纳米纤维在体外和体内在临界大小的兔颅骨缺损中均显示出显着的骨再生能力。，比负载骨髓MSC（BMSC）/ PHBV电纺丝纳米纤维更大。另外，BMSCs / PHBV电纺丝纳米纤维在手术前需要与BMSCs一起培养一段时间，而腺苷/ PHBV电纺丝纳米纤维可以直接植入。迄今为止，很少有研究评估掺有腺苷的电纺纳米纤维的骨再生能力。使用简单的制造工艺并具有与天然细胞外基质（ECM）相似的结构，静电纺丝的腺苷/ PHBV纳米纤维表现出出色的生物相容性和成骨能力。此外，腺苷价格便宜，易于获得和存储，因此在骨组织工程应用中具有巨大的实用潜力。