当前位置: X-MOL 学术Clin. Proteom. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Age-associated changes in human tear proteome.
Clinical Proteomics ( IF 2.8 ) Pub Date : 2019-03-30 , DOI: 10.1186/s12014-019-9233-5
Janika Nättinen 1 , Antti Jylhä 1 , Ulla Aapola 1 , Petri Mäkinen 2 , Roger Beuerman 1, 3, 4 , Juhani Pietilä 2 , Anu Vaajanen 5 , Hannu Uusitalo 1, 5
Affiliation  

Background Prevalence of many eye and ocular surface diseases increases with age. While the clinical characteristics and pathophysiologic mechanisms of these conditions are often either known or extensively studied, the effects of normal aging on tear film and ocular surface have not been as widely researched. Methods In order to examine the effects of aging on tear fluid proteomics, tear fluid samples were collected preoperatively from 115 subjects undergoing strabismus or refractive surgery using glass microcapillary tubes. In addition to their refractive error or strabismus, the subjects did not have any other current, known eye diseases. The non-pooled samples were analysed using NanoLC-TripleTOF implementing a sequential window acquisition of all theoretical fragment ion spectra mass spectrometry, resulting in quantified data of 849 proteins. Results According to correlation results, 17 tear proteins correlated significantly with increased age and many of these proteins were connected to inflammation, immune response and cell death. According to enrichment analysis, growth and survival of cells decreased while immune response and inflammation increased with aging. We also discovered several well-known, activated and inhibited upstream regulators, e.g. NF-κB, which has been previously connected to aging in numerous previous studies. Conclusions Overall, the results show the common age-dependent alterations in tear fluid protein profile, which demonstrate similar age-associated alterations of biological functions previously shown in other tissue and sample types.

中文翻译:

人类泪液蛋白质组的年龄相关变化。

背景 许多眼睛和眼表疾病的患病率随着年龄的增长而增加。虽然这些病症的临床特征和病理生理机制通常是已知的或广泛研究的,但正常老化对泪膜和眼表的影响尚未得到广泛研究。方法 为了检查衰老对泪液蛋白质组学的影响,术前使用玻璃微毛细管从 115 名接受斜视或屈光手术的受试者中收集泪液样本。除了屈光不正或斜视外,受试者没有任何其他当前已知的眼部疾病。使用 NanoLC-TripleTOF 对非混合样品进行分析,对所有理论碎片离子光谱质谱进行顺序窗口采集,得到 849 种蛋白质的量化数据。结果 根据相关结果,17 种泪液蛋白与年龄增加显着相关,其中许多蛋白与炎症、免疫反应和细胞死亡有关。根据富集分析,随着年龄的增长,细胞的生长和存活率下降,而免疫反应和炎症增加。我们还发现了几个众所周知的、激活和抑制的上游调节因子,例如 NF-κB,在之前的许多研究中,它与衰老有关。结论 总体而言,结果显示了泪液蛋白谱中常见的年龄依赖性变化,这证明了以前在其他组织和样本类型中显示的与年龄相关的生物学功能的类似变化。17 种泪液蛋白与年龄增长显着相关,其中许多蛋白质与炎症、免疫反应和细胞死亡有关。根据富集分析,随着年龄的增长,细胞的生长和存活率下降,而免疫反应和炎症增加。我们还发现了几个众所周知的、激活和抑制的上游调节因子,例如 NF-κB,在之前的许多研究中,它与衰老有关。结论 总体而言,结果显示了泪液蛋白谱中常见的年龄依赖性变化,这证明了以前在其他组织和样本类型中显示的与年龄相关的生物学功能的类似变化。17 种泪液蛋白与年龄增长显着相关,其中许多蛋白质与炎症、免疫反应和细胞死亡有关。根据富集分析,随着年龄的增长,细胞的生长和存活率下降,而免疫反应和炎症增加。我们还发现了几个众所周知的、激活和抑制的上游调节因子,例如 NF-κB,在之前的许多研究中,它与衰老有关。结论 总体而言,结果显示了泪液蛋白谱中常见的年龄依赖性变化,这证明了以前在其他组织和样本类型中显示的与年龄相关的生物学功能的类似变化。细胞的生长和存活率下降,而免疫反应和炎症随着衰老而增加。我们还发现了几个众所周知的、激活和抑制的上游调节因子,例如 NF-κB,在之前的许多研究中,它与衰老有关。结论 总体而言,结果显示了泪液蛋白谱中常见的年龄依赖性变化,这证明了以前在其他组织和样本类型中显示的与年龄相关的生物学功能的类似变化。细胞的生长和存活率下降,而免疫反应和炎症随着衰老而增加。我们还发现了几个众所周知的、激活和抑制的上游调节因子,例如 NF-κB,在之前的许多研究中,它与衰老有关。结论 总体而言,结果显示了泪液蛋白谱中常见的年龄依赖性变化,这证明了以前在其他组织和样本类型中显示的与年龄相关的生物学功能的类似变化。
更新日期:2020-04-22
down
wechat
bug