Rbfox RNA-binding proteins are expressed in the adult mammalian brain and are required for proper brain development and function. Studies in mice and humans have implicated Rbfox1/RBFOX1 in autism, neuronal excitation and epilepsy, and Rbfox2/RBFOX2 in cerebellar development. The zebrafish has emerged as a prominent model system for brain study, possessing neuroanatomical conservation with mammals and an extensive capacity for adult neurogenesis and plasticity. In this study, we characterize Rbfox1l and Rbfox2 expression in the adult zebrafish brain. While Rbfox2 is expressed broadly, Rbfox1l is expressed in restricted populations of neurons in the dorsal telencephalon and cerebellum. In the dorsal telencephalon, Rbfox1l is expressed in a specific population of neurons spanning Dm and Dc regions. In the cerebellum, Rbfox1l and Rbfox2 are expressed in the Purkinje cell layer, reminiscent of Rbfox1 and Rbfox2 expression in the mammalian cerebellum. Our findings motivate future studies of Rbfox function in the zebrafish brain.

Rbfox RNA结合蛋白在成年哺乳动物的大脑中表达，是正常大脑发育和功能所必需的。小鼠和人类研究表明，Rbfox1 / RBFOX1与自闭症，神经元兴奋和癫痫有关，而Rbfox2 / RBFOX2与小脑发育有关。斑马鱼已经成为大脑研究的重要模型系统，对哺乳动物具有神经解剖学保护能力，并具有成年神经发生和可塑性的广泛能力。在这项研究中，我们表征了成年斑马鱼脑中Rbfox11和Rbfox2的表达。尽管Rbfox2广泛表达，但Rbfox11在背侧末梢脑和小脑的有限神经元群体中表达。在背端脑中，Rbfox11在跨越Dm和Dc区域的特定神经元群体中表达。在小脑里 Rbfox11和Rbfox2在Purkinje细胞层中表达，让人联想到哺乳动物小脑中Rbfox1和Rbfox2的表达。我们的发现激发了斑马鱼大脑中Rbfox功能的未来研究。