Plant zygotes usually undergo asymmetrical cell division, giving rise to the formation of two daughter cells with distinct developmental cell fate. The small apical cell will develop into the major part of embryo proper, whereas the larger basal cell will divide to form a transient suspensor. Thus, the apical and basal cell lineages are an excellent model to study cell fate determination in relation to zygote polarity. However, the molecular mechanism underlying the differentiation of two distinct cell lineages is not yet understood, possibly due to the technique limitations. Previously, we have established a protocol for isolating apical cell and basal cell for cDNA library construction in tobacco. However, the method for isolating tiny Arabidopsis embryos has long been considered much more difficult. Here, we present a detailed protocol for isolating early Arabidopsis proembryos and separating apical and basal cell lineages of proembryos, which allow us to establish cell lineage-specific transcriptomes of early proembryos.

中文翻译：

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分离早期胚的活的顶端和基底细胞谱系用于转录组分析。

植物合子通常经历不对称的细胞分裂，从而形成具有不同发育细胞命运的两个子细胞的形成。小的顶端细胞将发育成胚胎固有的主要部分，而较大的基底细胞将分裂形成一个短暂的感受器。因此，顶端和基底细胞谱系是研究与合子极性相关的细胞命运确定的极好模型。然而，可能由于技术限制，尚未了解两种不同细胞谱系分化的分子机制。以前，我们已经建立了一种分离烟草中顶端细胞和基底细胞的协议，用于构建cDNA文库。但是，分离微小拟南芥的方法长期以来，人们一直认为胚胎要困难得多。在这里，我们提出了一个详细的协议，用于分离早期拟南芥原胚和分离顶胚和顶端细胞谱系，这使我们能够建立早期胚芽的细胞谱系特异性转录组。