ABSTRACT Long non-coding RNAs (lncRNAs) have been found to participate in the regulation of human spermatogenic cell development. However, little is known about the abnormal expression of lncRNAs associated with spermatogenic failure and their molecular mechanisms. Using lncRNA microarray of testicular tissue for male infertility and bioinformatics methods, we identified the relatively conserved lncRNA Gm2044 which may play important roles in non-obstructive azoospermia. The UCSC Genome Browser showed that lncRNA Gm2044 is the miR-202 host gene. This study revealed that lncRNA Gm2044 and miR-202 were significantly increased in non-obstructive azoospermia of spermatogonial arrest. The mRNA and protein levels of Rbfox2, a known direct target gene of miR-202, were regulated by lncRNA Gm2044. Furthermore, the miR-202-Rbfox2 signalling pathway was shown to mediate the suppressive effects of lncRNA Gm2044 on the proliferation of human testicular embryonic carcinoma cells. Understanding of the molecular signalling pathways for lncRNA-regulated spermatogenesis will provide new clues into the pathogenesis and treatment of patients with male infertility.

中文翻译：

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上调 lncRNA Gm2044 通过充当 miR-202 宿主基因抑制雄性生殖细胞发育

摘要 长链非编码 RNA (lncRNAs) 已被发现参与人类生精细胞发育的调控。然而，关于与生精衰竭相关的 lncRNA 的异常表达及其分子机制知之甚少。使用用于男性不育症的睾丸组织 lncRNA 微阵列和生物信息学方法，我们确定了相对保守的 lncRNA Gm2044，它可能在非梗阻性无精子症中起重要作用。UCSC Genome Browser 显示 lncRNA Gm2044 是 miR-202 宿主基因。该研究表明，lncRNA Gm2044 和 miR-202 在精原细胞停滞的非梗阻性无精子症中显着增加。Rbfox2（一种已知的 miR-202 直接靶基因）的 mRNA 和蛋白质水平受 lncRNA Gm2044 调节。此外，miR-202-Rbfox2信号通路介导lncRNA Gm2044对人睾丸胚胎癌细胞增殖的抑制作用。了解 lncRNA 调控的精子发生的分子信号通路将为男性不育患者的发病机制和治疗提供新的线索。