Determination of genetic effects of SERPINA3 on important growth traits in beef cattle.,Animal Biotechnology

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Determination of genetic effects of SERPINA3 on important growth traits in beef cattle.
Animal Biotechnology ( IF 1.7 ) Pub Date : 2019-03-31 , DOI: 10.1080/10495398.2018.1560306
Shi-Zhen Yang ₁ , Hua He _{1,

2} , Zi-Jing Zhang ₃ , Hui Niu ₄ , Fu-Ying Chen ₃ , Yi-Fan Wen ₁ , Jia-Wei Xu ₁ , Rui-Hua Dang ₁ , Xian-Yong Lan ₁ , Chu-Zhao Lei ₁ , Hong Chen ₁ , Bi-Zhi Huang ₅ , Yong-Zhen Huang ₁

Affiliation

Serine protease inhibitor protein 3 (serpin peptidase inhibitor, clade A, member 3, SERPINA3) is a member of the serpin superfamily, probably related to the yield and quality of muscle. This study focuses on the relationship between SERPINA3 gene polymorphism and growth traits in beef cattle. The study first uses sequencing pooled DNA samples (Pool-Seq), PCR-RFLP and Tetra-primer ARMS-PCR techniques to determine the genetic polymorphisms of SERPINA3 in 765 beef cattle. Then, the polymorphic loci were correlated with the growth characters of cattle. Five SNPs (SNP1:A-648G, SNP2:T6496A, SNP3:G2495A, SNP4:T2595A, SNP5:A2615G) were found, located in the promoter, introns 5 and SNP 3, 4, 5 were in exons 2, respectively. The observed He was from 0.44 to 0.5, Ne were approaching 2 (1.78 to 2.00). The maximum and minimum PIC (polymorphism information content) values were 0.37 and 0.34, respectively. The association analysis results showed that the SNPs had a significant height in the chest girth and body length. (p < 0.05 or p < 0.01). This will provide important information for the rapid breeding of Chinese yellow cattle and the establishment of a molecular genetic marker database.

更新日期：2020-04-23

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