ABSTRACT Carbohydrates present on cell surfaces mediate cell behavior through interactions with other biomolecules. Due to their structural complexity, diversity, and heterogeneity, it is difficult to fully characterize a variety of carbohydrates and their binding partners. As a result, novel technologies for glycomics applications have been developed, including carbohydrate microarrays and label-free detection methods. In this paper, we report using the combination of oligosaccharide microarrays and the label-free oblique-incidence reflectivity difference microscopy for real-time characterization of oligosaccharide-binding proteins. Aminated human milk oligosaccharides were immobilized on epoxy-coated glass substrates as microarrays for reactions with Family 1 of solute-binding proteins from Bifidobacterium longum subsp. infantis (B. infantis). Binding affinities of these protein–oligosaccharide interactions showed preferences of Family 1 of solute-binding proteins to host glycans, which helps in characterizing the complex process of human milk oligosaccharides foraging by B. infantis.

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使用碳水化合物微阵列和基于椭偏仪的生物传感器实时、无标记地表征寡糖结合蛋白

摘要 存在于细胞表面的碳水化合物通过与其他生物分子的相互作用来介导细胞行为。由于它们的结构复杂性、多样性和异质性，很难完全表征各种碳水化合物及其结合伙伴。因此，已经开发了用于糖组学应用的新技术，包括碳水化合物微阵列和无标记检测方法。在本文中，我们报告使用寡糖微阵列和无标记斜入射反射率差异显微镜的组合对寡糖结合蛋白进行实时表征。胺化人乳寡糖被固定在环氧树脂涂层的玻璃基板上作为微阵列，用于与来自长双歧杆菌亚种的溶质结合蛋白家族 1 反应。婴儿 (B. infantis)。