Sporothrix globosa is an important clinical pathogen in the Sporothrix complex, which is causing sporotrichosis. S. globosa is distributed worldwide, especially in Asia. The transmission medium of S. globosa is mainly contaminated soil or decaying vegetation, and the infection usually caused by transcutaneous trauma, through which the fungal conidia or yeast cells enter the host. Although the clinical manifestations of sporotrichosis caused by S. globosa is always benign, there have been several outbreaks worldwide. In this study, we established a novel real-time polymerase chain reaction (PCR) method based on the internal transcribed spacer (ITS) sequence for the identification of S. globosa. The assay was further evaluated by clinical specimens obtained from patients of sporotrichosis. The sensitivity and specificity of the real-time PCR method was both 100%. The detection limit was 10 fg. The positive detection rate for 30 clinical specimens, which were confirmed infected by S. globosa, was 100%. The real-time PCR method established in this paper is a rapid, sensitive and specific method for the identification of S. globosa. It can detect S. globosa in clinical specimen from patients with sporotrichosis, which is helpful for fast clinical diagnosis.

中文翻译：

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实时聚合酶链反应的开发和评估，用于快速诊断由球形孢子虫引起的孢子虫病。

球形Sporothrix globosa是Sporothrix复合体中重要的临床病原体，会引起孢子体增生。S. globosa分布于世界各地，尤其是在亚洲。球形链球菌的传播媒介主要是被污染的土壤或腐烂的植被，感染通常是由经皮创伤引起的，真菌分生孢子或酵母细胞通过该创伤进入宿主。尽管由球形链球菌引起的孢子体增生的临床表现始终是良性的，但全球范围内已有数起暴发。在这项研究中，我们建立了一种基于内部转录间隔区（ITS）序列的新型实时聚合酶链反应（PCR）方法，用于鉴定球形链球菌。通过从孢子虫病患者获得的临床标本进一步评估该测定法。实时荧光定量PCR方法的灵敏度和特异性均为100％。检出限为10 fg。证实已被球菌感染的30个临床标本的阳性检出率为100％。本文建立的实时荧光定量PCR方法是一种快速，灵敏，特异的鉴定球形葡萄球菌的方法。可以检测出孢子虫病患者的临床标本中的链球菌，有助于快速的临床诊断。