ABSTRACT Mycoplasma pneumoniae is a human respiratory tract pathogen causing chronic bronchitis and atypical or “walking” pneumonia. The major surface protein P1 must form complexes with proteins P30 and P40/P90 to function in receptor binding and gliding motility, and variability in P1 and P40/P90 distinguishes the two major M. pneumoniae genotypes. Strains belonging to each genotype can be differentiated with high sensitivity and specificity by utilizing surface-enhanced Raman spectroscopy on silver nanorod arrays. Here, we used the variable selection method of variable importance in projection (VIP) to identify Raman bands important in M. pneumoniae strain classification. Furthermore, VIP analysis of mutants lacking P40/P90, or P1 and P40/P90, correlated certain Raman bands important in distinguishing genotypes, with specific mycoplasma surface protein composition and presentation. Variable selection, and its correlation with specific mycoplasma surface components, is an important next step in developing this platform for M. pneumoniae detection and genotyping.

中文翻译：

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表面增强拉曼光谱分析肺炎支原体菌株的变量选择和生物标志物相关性

摘要肺炎支原体是一种人类呼吸道病原体，可引起慢性支气管炎和非典型或“行走”肺炎。主要表面蛋白 P1 必须与蛋白 P30 和 P40/P90 形成复合物才能在受体结合和滑动运动中发挥作用，P1 和 P40/P90 的变异性区分了两种主要的肺炎支原体基因型。通过利用银纳米棒阵列上的表面增强拉曼光谱，可以以高灵敏度和特异性区分属于每个基因型的菌株。在这里，我们使用投影中变量重要性的变量选择方法 (VIP) 来识别在肺炎支原体菌株分类中重要的拉曼谱带。此外，对缺乏 P40/P90 或 P1 和 P40/P90 的突变体的 VIP 分析，关联了某些对区分基因型很重要的拉曼谱带，具有特定的支原体表面蛋白组成和表现形式。变量选择及其与特定支原体表面成分的相关性是开发该肺炎支原体检测和基因分型平台的重要下一步。