Background: Disruption of the balance between the production of ROS and their removal through enzymatic and non-enzymatic (antioxidant) processes has been proposed as a new mechanism in the pathology of polycystic ovary syndrome (PCOS). Evidence from animal models of PCOS (prenatally androgenized sheep) has suggested that treatment with insulin sensitizers, but not antiandrogens, can reduce increases in ROS.
Materials and methods: In the present study, we investigated the effects of neonatal treatment with a gonadotropin-releasing hormone (GnRH) agonist (leuprolide acetate) on prenatally androgenized sheep with testosterone propionate to determine its impact on oxidative stress molecules (ferric reducing antioxidant power [FRAP], advanced oxidation protein product [AOPP], nitric oxide [NOx], albumin) at 8, 12, and 18 months of age.
Results: Androgenized ewes (but not leuprolide-treated ewes) showed reduced total cholesterol levels associated with a decrease in the ratio of visceral to subcutaneous adiposity (adjusted to abdominal area) as determined by computed tomography. In androgenized ewes at 12 months of age, an increase in subcutaneous fat and relative decrease in the visceral fat compartment did not affect the expression of REDOX markers. At 18 months of age, however, the levels of NOx metabolites decreased in androgenized animals, but remained close to normal in ewes subjected to neonatal treatment with leuprolide acetate. Other oxidative stress parameters (FRAP, AOPP, albumin) did not vary among groups.
Conclusion: Our results demonstrate that the GnRH agonist leuprolide (as a single dose after birth) had weak effects on markers of the oxidative stress balance.



中文翻译：

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新生儿用 GnRH 激动剂治疗后产前雄激素化绵羊的氧化应激和生化标志物。

背景：已提出破坏 ROS 的产生与通过酶促和非酶促（抗氧化）过程去除它们之间的平衡作为多囊卵巢综合征 (PCOS) 病理学中的一种新机制。来自 PCOS（产前雄激素化绵羊）动物模型的证据表明，使用胰岛素增敏剂而非抗雄激素治疗可以减少 ROS 的增加。
材料和方法：在本研究中，我们研究了使用促性腺激素释放激素 (GnRH) 激动剂（醋酸亮丙瑞林）对产前雄性化绵羊丙酸睾酮进行新生儿治疗的影响，以确定其对氧化应激分子（铁降低抗氧化能力 [FRAP]、 8、12 和 18 个月大时的高级氧化蛋白质产物 [AOPP]、一氧化氮 [NOx]、白蛋白。
结果：通过计算机断层扫描确定，雄激素化的母羊（但不是亮丙瑞林治疗的母羊）显示总胆固醇水平降低，这与内脏与皮下肥胖（调整到腹部面积）的比率降低有关。在 12 月龄的雄激素化母羊中，皮下脂肪的增加和内脏脂肪室的相对减少不影响 REDOX 标志物的表达。然而，在 18 个月大时，雄激素化动物的 NOx 代谢物水平下降，但在接受醋酸亮丙瑞林处理的母羊中仍接近正常水平。其他氧化应激参数（FRAP、AOPP、白蛋白）在各组之间没有差异。
结论：我们的研究结果表明，GnRH 激动剂亮丙瑞林（作为出生后的单剂量）对氧化应激平衡的标志物影响较弱。