INTRODUCTION Perinatal maternal separation stress (PMSS) induces brain lipid peroxidation and reduction in endogenous antioxidants. The present study was designed to assess the brain oxidative stress (MDA) and protein thiol levels through various stages of aging in PMSS rat pups supplemented with choline with docosahexaenoic acid (DHA) or Clitoria ternatea (Linn) aqueous root extract (CTR). MATERIAL AND METHODS Study groups, control, PMSS, PMSS + choline with DHA, PMSS + CTR (n = 6/group) were included in the study. Pups of PMSS groups were separated from their mothers for a period of 6 h/day for 30 days. PMSS + supplemented groups were treated as appropriate during the same period. Rats were sacrificed on day 30, 60, 90, 210 and 360. Brains were processed for MDA and protein thiol levels. RESULTS Brain MDA levels were significantly increased in PMSS rats at day 30, 60 (p < 0.001), 90 (p < 0.01) and attenuated in PMSS pups supplemented with choline with DHA and CTR at day 30, 60 (p < 0.01), 90 (p < 0.01, p < 0.05) and 360 (p < 0.001) when compared to the same in age-matched controls and PMSS rats, respectively. Alternatively, brain protein thiol levels in PMSS rats were reduced in all age groups when compared to the same in age-matched controls. A significant increase in brain thiol levels was observed in supplemented groups at day 60 (p < 0.01) and 210 (p < 0.01, p < 0.05) when compared to the same in age-matched PMSS rats. CONCLUSIONS PMSS causes enhanced brain lipid peroxidation (MDA levels) and reduces endogenous antioxidants. Supplementation of choline and DHA or CTR during PMSS in rats persistently attenuates brain oxidative stress through aging.

中文翻译：

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在围产期的母鼠分离的幼崽中补充胆碱和二十二碳六烯酸或Clitoria ternatea水性根提取物，通过衰老持续减轻脑部氧化应激。

引言围产期母体分离应激（PMSS）诱导脑脂质过氧化并减少内源性抗氧化剂。本研究旨在评估在补充了胆碱，二十二碳六烯酸（DHA）或Clitoria ternatea（Linn）水根提取物（CTR）的PMSS大鼠幼仔中，通过衰老的各个阶段来评估大脑的氧化应激（MDA）和蛋白质硫醇水平。材料与方法研究组，对照组，PMSS，PMSS +胆碱加DHA，PMSS + CTR（n = 6 /组）纳入研究。将PMSS组的幼犬从母亲那里分离出来，持续6天/天，持续30天。同期对PMSS +补充组进行了适当治疗。在第30、60、90、210和360天处死大鼠。对大脑进行MDA和蛋白质硫醇水平处理。结果PMSS大鼠在第30、60（p <0.001），90（p <0.01）天的脑MDA水平显着升高，而在补充DHA和CTR胆碱的PMSS幼犬在30、60（p <0.01）时脑MDA水平降低，与年龄匹配的对照组和PMSS大鼠相比分别为90（p <0.01，p <0.05）和360（p <0.001）。或者，与年龄匹配的对照组相比，所有年龄组的PMSS大鼠脑蛋白硫醇水平均降低。与年龄匹配的PMSS大鼠相比，补充组在第60天（p <0.01）和210天（p <0.01，p <0.05）观察到脑硫醇水平显着增加。结论PMSS可增强脑脂质过氧化（MDA水平）并减少内源性抗氧化剂。