The aim of this study was to investigate the effect and mechanism of chronic intermittent hypoxia (CIH) on cerebral injury using the ischemia-reperfusion rat model. In total, 36 SD rats were divided into three groups: pseudo-surgery group (sham group), ischemia-reperfusion (IR) group (CIR group), and CIH intervention group (CIH group). The IR model was established using the suture-occluded method. CIH intervention was performed starting at 12 weeks prior to the establishment of the IR model for rats in the CIH group. Ultra-microstructure was examined using an electron microscope. Expression of intercellular cell adhesion molecule-1 (ICAM-1) and vascular endothelial growth factor (VEGF) in rat brain tissue was evaluated by immunohistochemical methods and western blot assays. The neurological deficit scores of rats in the CIH group were higher than those in the CIR group (p < 0.05). Using electron microscopy, we observed more severe edema around the capillaries of the rat brain that resulted in more pressure on the vascular wall of the capillaries in the CIH group compared to the CIR group. The expression of ICAM-1 and VEGF in rat brain tissue was rare in the sham group, but was significantly elevated in the CIR group (p < 0.05) and even higher in the CIH group, compared to the CIR group. Hence, the brain injury in ischemia-reperfusion rat models following CIH intervention may be related to the increased expression of ICAM-1 and VEGF.

中文翻译：

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慢性间歇性缺氧对缺血再灌注大鼠模型中细胞间黏附分子-1和血管内皮生长因子表达的影响。

这项研究的目的是使用缺血再灌注大鼠模型研究慢性间歇性缺氧（CIH）对脑损伤的作用和机制。总共36只SD大鼠分为三组：假手术组（假手术组），缺血再灌注（IR）组（CIR组）和CIH干预组（CIH组）。采用缝合法建立IR模型。CIH干预是在建立CIH组大鼠的IR模型之前的12周开始进行的。使用电子显微镜检查超微结构。通过免疫组织化学方法和蛋白质印迹分析评估大鼠脑组织中细胞间粘附分子-1（ICAM-1）和血管内皮生长因子（VEGF）的表达。CIH组大鼠的神经功能缺损评分高于CIR组（p <0.05）。使用电子显微镜，我们观察到与CIR组相比，CIH组的大鼠大脑毛细血管周围出现了更严重的水肿，导致对毛细血管壁的压力更大。与假手术组相比，假手术组大鼠脑组织中ICAM-1和VEGF的表达很少，但在CIR组中明显升高（p <0.05），在CIH组中甚至更高。因此，CIH干预后缺血再灌注大鼠模型中的脑损伤可能与ICAM-1和VEGF的表达增加有关。我们观察到，与CIR组相比，CIH组的大鼠大脑毛细血管周围出现了更严重的水肿，导致对毛细血管血管壁的压力更大。与假手术组相比，假手术组大鼠脑组织中ICAM-1和VEGF的表达很少，但在CIR组中明显升高（p <0.05），在CIH组中甚至更高。因此，CIH干预后缺血再灌注大鼠模型中的脑损伤可能与ICAM-1和VEGF的表达增加有关。我们观察到，与CIR组相比，CIH组的大鼠大脑毛细血管周围出现了更严重的水肿，导致对毛细血管血管壁的压力更大。与假手术组相比，假手术组大鼠脑组织中ICAM-1和VEGF的表达很少，但在CIR组中明显升高（p <0.05），在CIH组中甚至更高。因此，CIH干预后缺血再灌注大鼠模型中的脑损伤可能与ICAM-1和VEGF的表达增加有关。