Caulobacter crescentus is a model organism for the integrated circuitry that runs a bacterial cell cycle. Full discovery of its essential genome, including non-coding, regulatory and coding elements, is a prerequisite for understanding the complete regulatory network of a bacterial cell. Using hyper-saturated transposon mutagenesis coupled with high-throughput sequencing, we determined the essential Caulobacter genome at 8 bp resolution, including 1012 essential genome features: 480 ORFs, 402 regulatory sequences and 130 non-coding elements, including 90 intergenic segments of unknown function. The essential transcriptional circuitry for growth on rich media includes 10 transcription factors, 2 RNA polymerase sigma factors and 1 anti-sigma factor. We identified all essential promoter elements for the cell cycle-regulated genes. The essential elements are preferentially positioned near the origin and terminus of the chromosome. The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species.

中文翻译：

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细菌的基本基因组。

Caulobacter crescentus 是运行细菌细胞周期的集成电路的模式生物。全面发现其基本基因组，包括非编码、调控和编码元件，是了解细菌细胞完整调控网络的先决条件。使用超饱和转座子诱变结合高通量测序，我们以 8 bp 的分辨率确定了必需的 Caulobacter 基因组，包括 1012 个必需的基因组特征：480 个 ORF、402 个调控序列和 130 个非编码元件，包括 90 个未知功能的基因间片段. 在富培养基上生长的基本转录回路包括 10 个转录因子、2 个 RNA 聚合酶 sigma 因子和 1 个抗 sigma 因子。我们鉴定了细胞周期调节基因的所有必需启动子元件。基本元素优先位于染色体的起点和终点附近。这里使用的高分辨率策略适用于大类细菌物种的高通量、全基因组重要性研究和大规模遗传扰动实验。